贵州省2017-2018年非伤寒沙门菌临床分离株耐药及分子分型研究  

Antimicrobial resistance and molecular typing of non-typhoid Salmonella clinical isolates from Guizhou Province in 2017-2018

作　　者：龙利 游旅[2] 韦小瑜 李世军[2] 王丹[2] 黄俊飞[2] 童毅 郭华[2] 王铭[2] 汪俊华 LONG Li;YOU Lü;WEI Xiao-yu;LI Shi-jun;WANG Dan;HUANG Jun-fei;TONG Yi;GUO Hua;WANG Ming;WANG Jun-hua(School of Public Health,the key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,Guiyang 550025,China;Guizhou Provincial Center for Disease Control and Prevention,Guiyang 550004,China)

机构地区：[1]贵州医科大学公共卫生学院,环境污染与疾病监控教育部重点实验室,贵阳550025 [2]贵州省疾病预防控制中心,贵阳550004

出　　处：《中国人兽共患病学报》2021年第7期603-610,共8页Chinese Journal of Zoonoses

基　　金：贵州省科技支撑项目(黔科合支撑[2020]4Y143号,黔科合支撑[2020]4Y216号);贵州省科技基金计划项目(黔科合基础[2017]1094);贵州省卫健委科技项目(gzwjkj2016-1-003);贵州省科技创新人才团队(黔科合平台人才[2018]5606)。

摘　　要：目的了解贵州省2017-2018年非伤寒沙门菌(NTS)的血清型分布、耐药情况与脉冲凝胶电泳(PFGE)分子分型特征。方法对分离自贵州省临床病例的191株非伤寒沙门菌菌株进行生化鉴定、血清型分型,微量肉汤稀释法测定16种抗生素的最小抑菌浓度,PFGE进行分子分型分析。结果 191株非伤寒沙门菌分出18种血清型,鼠伤寒沙门菌(45.5%,87/191)和肠炎沙门菌(29.3%,56/191)为主要血清型。对所测抗生素产生耐药的菌株占96.8%(185/191),对磺胺异恶唑、链霉素、氨苄西林、四环素、多西环素耐药率较高,均超过60.0%。对头孢曲松和环丙沙星耐药率分别为7.9%(15/191)和13.1%(25/191)。多重耐药率为84.3%(161/191),共有69种耐药谱,主要耐药谱型为氨苄西林+链霉素+磺胺异恶唑+萘啶酸和氨苄西林+氯霉素+链霉素+磺胺异恶唑+四环素+多西环素+复方新诺明。87株鼠伤寒沙门菌共分为60种带型,优势型别为JPXX01.GZ0047(12.6%,11/87)。56株肠炎沙门菌共分为21种带型,优势型别为JEGX01.GZ0005(23.3%,13/56)。结论贵州省NTS主要血清型为鼠伤寒沙门菌和肠炎沙门菌,多重耐药现象严重,PFGE带型总体呈多样性,部分带型与耐药表型有一定的关联性。To understand the serotype distribution,antimicrobial resistance and pulsed field gel electrophoresis(PFGE)molecular typing characteristics of non-typhoid salmonella(NTS)in Guizhou Province,we identified 191 NTS clinical isolates through biochemical methods and performed serotyping with the micro broth dilution method against 16 antimicrobial agents;molecular typing was detected by PFGE.A total of 191 clinical isolates were identified as 18 serotypes.The dominant serotypes were Salmonella Typhimurium(45.5%,87/191)and Salmonella Enteritidis(29.3%,56/191).A total of 96.8%(185/191)of the isolates showed resistance to the antimicrobial agents tested.Resistance to sulfaisoxazole,streptomycin,ampicillin,tetracycline and doxycycline reached as high as>60.0%.Resistance to ceftriaxone and ciprofloxacin was found in 7.9%(15/191)and 13.1%(25/191)of the isolates,respectively.Approximately 84.3%(161/191)of the isolates were identified as multi-drug resistant(MDR).Sixty-nine antimicrobial resistance profiles were observed,and the major antimicrobial resistance profiles were ampicillin+streptomycin+sulfamethoxazole+nalidixic,and ampicillin+chloramphenicol+streptomycin+sulfamethoxazole+tetracycline+doxycycline+trimethoprim/sulfamethoxazole.Eighty-seven S.Typhimurium isolates were divided into 60 PFGE types,and the dominant type was JPXX01.GZ0047(12.6%,11/87).Fifty-six S.Enteritidis isolates were divided into 21 PFGE types,and the dominant type was JEGX01.GZ0005(23.3%,13/56).S.Typhimurium and S.Enteritidis were the dominant serotypes of NTS isolates in Guizhou Province.The prevalence of MDR is currently concerningly high.The PFGE types of NTS isolates were highly genetic diverse,and some types were related to antimicrobial resistance profiles.

关 键 词：非伤寒沙门菌 耐药性 分子分型 

分 类 号：R378.2[医药卫生—病原生物学]