High performance liquid chromatographic separation of eight drugs collected in Chinese Pharmacopoeia 2010 on amylose ramification chiral stationary phase  

作　　者：Yan Wang Ying Zhou Chao Ma Beibei Yang Ru Feng Yiying Zhang Jie Fu Wenjing Chen Yupeng Sun Jingyi Ma Qiming Zhang Yulin Deng Yukui Zhang Wenyi He 

机构地区：[1]School of Life,Beijing Institute of Technology,Beijing 100081,China [2]State Key Laboratory of Bioactive Substances and Functions of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100050,China [3]National Institute for the Control of Pharmaceutical and Biological Products,Beijing 100050,China

出　　处：《Acta Pharmaceutica Sinica B》2012年第5期527-533,共7页药学学报（英文版）

摘　　要：The enantiomers separation of ei ght pharmaceutical racemates collected in Chinese Pharmacopoeia 2010(Ch.P2010),including nitrendipine,felodipine,omeprazole,praziquantel,sulpiride,clenbuterol hydrochloride,verapamil hydrochloride and chlorphenamine maleate,was performed on chiral stationary phase of amylose ramification by high performance liquid chromatography(HPLC)on Chiralpak AD-H column and Chiralpak AS-H column with the mobile phase consisted of isopropanol and n-hexane.The detection wavelength and the flow rate were set at 254 nm and 0.7 mL/min,respectively.The effects of proportion of organic additives,alcohol displacer and temperature on the separation were investigated.The results indicated that eight chiral drugs were separated on chiral stationary phase of amylase ramification in normal phase chroma tographic system.The chromatographic retention and resolution of enantiomers were adjusted by factors,including the changes of the concentration of alcohol displacer in mobile phase,organic alkaline modifier and column temperature.It was shown that the resolution was improved with reducing concentration of alcohol displacer.When the concentration of organic alkaline modifier was 0.2%,the resolution and the peak shape were fairly good.Most racemates mentioned above had the best resolution at column temperature of 25℃.The best temperature should be kept unchanged in the process of separation so as to obtain stable separation results.

关 键 词：Normal phase HPLC Chiral stationary phase Optical enantiomers Amylose ramification NITRENDIPINE FELODIPINE Omeprazole Praziquantel SULPIRIDE Clenbuterol hydrochloride Verapamil hydrochloride Chlorphenamine maleate 

分 类 号：O62[理学—有机化学]