Determination of ifenprodil by LC–MS/MS and its application to a pharmacokinetic study in healthy Chinese volunteers  

作　　者：Jing Yang Chengtao Lu Wei Song Jiankang Li Yi Ding Yanrong Zhu Jinyi Cao Likun Ding Yanyan Jia Aidong Wen 

机构地区：[1]Department of Pharmacy,Xijing Hospital,Fourth Military Medical University,Shanxi 710032,China

出　　处：《Acta Pharmaceutica Sinica B》2013年第3期180-184,共5页药学学报（英文版）

摘　　要：This paper reports the development and validation of an assay for ifenprodil based on liquid chromatography–tandem mass spectrometry(LC–MS/MS)and its application to a pharmacokinetic study involving single and multiple intravenous infusions to healthy Chinese volunteers.After sample preparation of plasma by liquid–liquid extraction with ethyl acetate,the analyte and internal standard,urapidil,were separated by reversed phase chromatography in a run time of 4 min and detected by positive ion electrospray ionization followed by multiple reaction monitoring of the precursor-to-product ion transitions at m/z 326.2-308.1 for ifenprodil and m/z 388.4-205.3 for IS.The assay was linear in the concentration range 0.2–50.0 ng/mL with recovery 476.4%.In the pharmacokinetic study of single intravenous infusions of 5,10 and 15 mg ifenprodil,peak plasma concentrations and areas under the plasma concentration–time curve were both linearly related to dose.In the pharmacokinetic study of multiple once daily intravenous infusions of 10 mg ifenprodil for 7 days,pharmacokinetic parameters were similar to those after the single dose showing that ifenprodil does not accumulate on repeated administration.

关 键 词：IFENPRODIL Liquid/liquid extraction LC–MS/MS PHARMACOKINETICS Intravenous infusion Human PLASMA 

分 类 号：O62[理学—有机化学]