A rapid and sensitive determination of paclitaxel in rat plasma by UPLC-MS/MS method: Application to a pharmacokinetic study  

作　　者：He Lian Jin Sun Tianhong Zhang 

机构地区：[1]Department of Biopharmaceutics,School of Pharmacy,Shenyang Pharmaceutical University,Mailbox 59#,No.103,Wenhua Road,Shenyang 110016,China

出　　处：《Asian Journal of Pharmaceutical Sciences》2013年第3期199-205,共7页亚洲药物制剂科学（英文）

基　　金：This work was financially supported from the National Nature Science Foundation of China(No.81173008);from the National Basic Research Program of China(973 Program)No.2009CB930300;from Project for Excellent Talents of Liaoning Province(No.LR20110028);from Program for New Century Excellent Talents in University(No.NCET-12-1015).

摘　　要：A rapid and sensitive method for quantitative determination of paclitaxel in rat plasmawas developed and validated by using ultra-performance liquid chromatography-tandemmass spectrometry (UPLC-MS/MS). Docetaxel was used as an internal standard anddiethyl ether was the liquideliquid extraction agent. Multiple reaction monitoring (MRM)mode via positive electrospray ionization (ESI) was applied to detect paclitaxel and IS at thetransitions m/z 854 / 286 and m/z 808.48 / 527.3, respectively. This method covered alinearity range from 5 to 5000 ng/ml, with the total run time of 3.0 min. In summary, a highthroughout UPLC-MS/MS method was successfully developed to measure paclitaxel in ratplasma and was applied to pharmacokinetic study after intravenous administration ofpaclitaxel.

关 键 词：PACLITAXEL UPLC-MS/MS method Liquideliquid extraction Pharmacokinetic study 

分 类 号：O62[理学—有机化学]