多发性骨髓瘤患者EB病毒检测及其临床意义  

Detection of Epstein-Barr Virus in Patients with Multiple Myeloma and Its Clinical Significance

作　　者：晏建国 罗萍[1] 胡飞[1] 欧阳贤凤[1] 李金凤[1] 朱文凤 郭瑛[1] YAN Jian-guo;LUO Ping;HU Fei;OUYANG Xian-feng;LI Jin-feng;ZHU Wen-feng;GUO Ying(Department of Hematology,First People's Hospital of Jiujiang City,Jiujiang 332000,Jiangxi,China)

机构地区：[1]九江市第一人民医院血液内科,江西九江332000

出　　处：《医学信息》2021年第15期86-88,共3页Journal of Medical Information

摘　　要：目的检测多发性骨髓瘤(MM)患者中EB病毒(EBV)感染情况,探讨其与MM的发生、发展及预后的关系。方法选取2012年5月~2020年2月在我院住院110例MM患者作为MM组,并选取同期健康者110名作为正常对照组,采用荧光定量PCR检测所有研究对象外周血细胞中EBV-DNA,比较两组EBV感染率及不同分型、不同分期(ISS分期)、不同危险度分层(mSMART)患者外周血细胞中EBV-DNA水平。结果MM组的EBV感染率为68.18%,高于对照组的8.18%(P<0.00);不同分型中EBV-DNA阳性表达率分别为IgG型57.78%、IgA型73.81%、IgD型66.67%、κ轻链型71.14%、入轻链型85.71%、不分泌型100.00%;不同分期(ISS分期)中EBV-DNA阳性表达率分别为Ⅰ期27.78%、Ⅱ期59.09%和Ⅲ期81.43%,且Ⅰ期EBV-DNA阳性表达率高于正常对照组,低于Ⅱ期和Ⅲ期(P<0.05),Ⅱ期EBV-DNA阳性表达率低于Ⅲ期(P<0.05);在不同危险度分层(mSMART)中EBV-DNA阳性表达率分别为低危组(43.75%)、中危组(69.77%)和高危组(88.57%),且低危组EBV-DNA阳性表达率高于正常对照组,低于中危组和高危组(P<0.05),中危组EBV-DNA阳性表达率低于高危组(P<0.05)。结论EBV感染与多发性骨髓瘤的发生可能密切相关,外周血EBV-DNA表达在多发性骨髓瘤患者临床分型、分期、危险度分层的存在差异,通过检测外周血EBV-DNA可作为临床上评估多发性骨髓瘤预后的重要参考指标,有一定的临床参考价值。Objective To detect Epstein-Barr virus(EBV)infection in patients with multiple myeloma(MM),and to explore its relationship with the occurrence,development and prognosis of MM.Methods A total of 110 patients with MM who were hospitalized in our hospital from May 2012 to February 2020 were selected as the MM group,and 110 healthy people during the same period were selected as the control group.Fluorescence quantitative PCR was used to detect EBV-DNA in peripheral blood cells of all subjects,the EBV infection rate and EBV-DNA levels in peripheral blood cells of patients with different types,different stages(ISS stages)and different risk stratification(mSMART)were compared between the two groups.Results The EBV infection rate in the MM group was 68.18%,which was higher than 8.18%in the control group(P<0.05);The positive expression rates of EBV-DNA in different types were 57.78%for IgG type,73.81%for IgA type,66.67%for IgD type,71.14%for k light chain type,85.71%for light chain type,and 100.00%for non-secreted type.The positive expression rates of EBV-DNA in different stages(ISS stages)were 27.78%in stageⅠ,59.09%in stageⅡand 81.43%in stageⅢ,respectively and the positive expression rate of EBV-DNA in stageⅠwas higher than that in the normal control group,and lower than in stagesⅡandⅢ(P<0.05).The positive expression rate of EBV-DNA in stageⅡwas lower than that in stageⅢ(P<0.05);The positive expression rates of EBV-DNA in different risk stratification(mSMART)were low-risk group(43.75%),intermediate-risk group(69.77%),and high-risk group(88.57%)and the positive expression rate of EBV-DNA in the low-risk group was higher than that of the normal control group,and lower than the middle-risk group and the high-risk group(P<0.05).The positive expression rate of EBV-DNA in the intermediate-risk group was lower than that in the high-risk group(P<0.05).Conclusion EBV infection may be closely related to the occurrence of multiple myeloma.The expression of EBV-DNA in peripheral blood has differences in the clinical

关 键 词：EB病毒 EBV-DNA 多发性骨髓瘤 

分 类 号：R733[医药卫生—肿瘤]