肝细胞癌组织中微小RNA-552、微小RNA-383的表达及其对患者术后5年不良预后结局的影响  

Expression of microRNA-552 and microRNA-383 in hepatocellular carcinoma and their influence on 5-year poor prognostic outcome

作　　者：赵娟[1] 李辉[2] 徐沛演 张成芳[2] 杜利军 曹天生[3] ZHAO Juan;LI Hui;XU Pei-yan;ZHANG Cheng-fang;DU Li-jun;CAO Tian-sheng(Department of Laboratory,Huadu District People′s Hospital of Guangzhou City,Guangdong Province,Guangzhou501800,China;Department of Internal Medicine,Huadu District People′s Hospital of Guangzhou City,Guangdong Province,Guangzhou501800,China;Department of Surgery,Huadu District People′s Hospital of Guangzhou City,Guangdong Province,Guangzhou501800,China)

机构地区：[1]广州市花都区人民医院检验科,广东广州501800 [2]广州市花都区人民医院内科,广东广州501800 [3]广州市花都区人民医院外科,广东广州501800

出　　处：《中国当代医药》2021年第21期23-28,F0003,共7页China Modern Medicine

基　　金：广东省广州市科技计划项目(202002030044);广东省广州市花都区人民医院院内科研基金项目(2020B03)。

摘　　要：目的检测微小RNA-552(miR-552)、微小RNA-383(miR-383)在肝细胞癌(HCC)组织中的表达情况,探讨miR-552与miR-383对HCC患者预后的影响。方法选取2011年2月—2014年2月于广州市花都区人民医院住院并行根治性切除术的68例HCC患者,取患者的HCC组织作为研究样本,取患者的癌旁正常组织(距肿瘤边缘>5 cm)作为对照样本。采用实时荧光定量聚合酶链反应(qRT-PCR)方法检测miR-552、miR-383的相对表达量;分别按HCC患者癌组织的miR-552、miR-383相对表达量高低,将其分为miR-552低表达组(miR-552相对表达量<1.28,n=26)、miR-552高表达组(miR-552相对表达量>1.28,n=42)、miR-383低表达组(miR-383相对表达量<0.83,n=29)、miR-383高表达组(miR-383相对表达量>0.83,n=39);χ2检验分析HCC组织中miR-552、miR-383表达与患者临床病理特征的关系;Kaplan-Meier生存曲线分析影响HCC术后预后的因素;COX回归分析影响HCC术后5年预后结局的因素。结果HCC组织中miR-552相对表达量高于癌旁正常组织,差异有统计学意义(P<0.05),miR-383相对表达量低于癌旁正常组织,差异有统计学意义(P<0.05);miR-552低表达组与miR-552高表达组患者的年龄、性别、肝硬化比例比较,差异无统计学意义(P>0.05);miR-552低表达组患者的组织高分化、肿瘤直径≥5 cm、发生淋巴结转移、发生远处转移、TNM分期Ⅲ~Ⅳ期、发生血管侵袭比例均低于miR-552高表达组,差异有统计学意义(P<0.05);miR-383低表达组与miR-383高表达组患者的年龄、性别、肝硬化、发生远处转移、发生血管侵袭比例比较,差异无统计学意义(P>0.05);miR-383低表达组患者的组织高分化、肿瘤直径≥5 cm、发生淋巴结转移、TNM分期Ⅲ~Ⅳ期比例高于miR-383高表达组,差异有统计学意义(P<0.05);miR-552低表达组患者的5年生存率高于miR-552高表达组,差异有统计学意义(P<0.05);miR-383低表达组患者的5年生存率低于miR-383高表达组,差异有统计学意�Objective To detect the expression of microRNA-552(miR-552)and microRNA-383(miR-383)in hepatocellular carcinoma(HCC)and to investigate the effect of miR-552 and miR-383 on the prognosis of HCC patients.Methods From February 2011 to February 2014,68 patients with HCC who were hospitalized and underwent radical resection in Huadu District People′s Hospital of Guangzhou City were selected,the HCC tissue of the patients was taken as the study sample,and the adjacent normal tissue(more than 5 cm from the edge of the tumor)was taken as the control sample,the relative expression of miR-552 and miR-383 was detected by real-time fluorescent quantitative polymerase chain reaction(qRT-PCR).According to the relative expression of miR-552 and miR-383 in HCC tissues,the patients were divided into miR-552 low expression group(miR-552 relative expression<1.28,n=26),miR-552 high expression group(miR-552 relative expression>1.28,n=42),miR-383 low expression group(miR-383 relative expression<0.83,n=29)and miR-383 high expression group(miR-383 relative expression>0.83,n=39).χ2 test was used to analyze the relationship between the expression of miR-552 and miR-383 and the clinicopathological characteristics of HCC.Kaplan-Meier survival curve was used to analyze the factors influencing the prognosis of HCC,and COX regression was used to analyze the factors influencing the 5-year postoperative outcome of HCC.Results The relative expression of miR-552 in HCC tissues was higher than that in adjacent normal tissues,the difference was statistically significant(P<0.05),the relative expression of miR-383 was lower than that in adjacent normal tissues,the difference was statistically significant(P<0.05);comparison of age,gender,the proportion of liver cirrhosis between miR-552 high expression group and miR-552 low expression group,the differences were not statistically significant(P>0.05),while the proportions of high differentiation,tumor diameter≥5 cm,lymph node metastasis,distant metastasis,TNM stageⅢ-Ⅳand vascular invasion in miR

关 键 词：肝细胞癌 微小RNA-552 微小RNA-383 预后 

分 类 号：R730.261[医药卫生—肿瘤]