^(13)C标记的PLFA法在微生物学中的应用及其在中药生态种植研究领域的展望  

Application of ^(13)C-labeled PLFA Analysis in Microbiology and Prospects in Ecological Planting of Chinese Medicinal Plants

作　　者：刘长征 姜晓琳 蔡启忠 杨全 周良云 LIU Chang-zheng;JIANG Xiao-lin;CAI Qi-zhong;YANG Quan;ZHOU Liang-yun(School of Traditional Chinese Medicine,Guangdong Pharmaceutical University,Key Laboratory for Production&Development of Cantonese Medicinal Materials,National Administration of Traditional Chinese Medicine,Guangzhou Comprehensive Experimental Station,Chinese Materia Medica Industry Technology System,Guangdong Provincial Research Center on Good Agricultural Practice&Comprehensive Agricultural Development Engineering Technology of Cantonese Medicinal Materials,Guangzhou 510006,China)

机构地区：[1]广东药科大学中药学院/国家中医药管理局岭南药材生产与开发重点研究室/国家中药材产业技术体系广州综合试验站/广东省南药规范化种植与综合开发工程技术研究中心,广东广州510006

出　　处：《中国现代中药》2021年第6期1103-1110,共8页Modern Chinese Medicine

基　　金：国家重点研发计划项目(2017YFC1700704);2017年广东省岭南中药材保护资金专项(粤财社〔2017〕60号);广东省普通高校青年创新人才类项目(2018KQNCX133);国家中药材产业技术体系建设专项(CARS-21)。

摘　　要：同位素^(13)C标记的磷脂脂肪酸(PLFA)法,可以追踪有微生物参与的碳循环过程,并且揭示微生物在此过程中的功能,PLFA法在微生物生态研究中已经被广泛应用。通过查阅国内外相关文献,归纳总结了^(13)C标记的PLFA法在微生物介导的植物-土壤碳循环中的应用,包括植物残体的微生物分解、土壤激发效应、丛枝菌根、光合作用产物的微生物利用及其在甲烷氧化菌、浮游植物等特定微生物上的应用。在此基础上,对其在中药生态种植研究领域进行展望,包括丛枝菌根增加药用植物抗逆性、缓解连作障碍和非药用部位堆肥还田。The isotope^(13)C-labeled phospholipid fatty acid(PLFA)analysis can track the carbon cycle involving microorganisms and reveal the functions of microorganisms in this process.This method has been widely used in microbial ecology.In this paper,through literature research,the application of^(13)C-labeled PLFA analysis in microorganisms-mediated plant-soil carbon cycle,including microbial decomposition of plant residues,soil priming effect,arbuscular mycorrhiza,and microbial utilization of photosynthesis products,and in specific microorganisms such as methane-oxidizing bacteria and phytoplankton,was summarized.On this basis,the potential of this method in ecological planting of Chinese medicinal plants was analyzed,such as(arbuscular mycorrhiza)enhancing the resistance of medicinal plants,alleviating continuous cropping obstacles,and turning non-medicinal parts into fertilizers by composting.

关 键 词：^(13)C 磷脂脂肪酸 微生物 碳循环 生态种植 

分 类 号：R286[医药卫生—中药学] S157.4[医药卫生—中医学]