lncRNA HOXA⁃AS2在胰腺癌组织中的表达及对细胞增殖和侵袭力的影响  

Expression of lncRNA HOXA⁃AS2 in pancreatic cancer tissues and its effect on cell proliferation and invasion

作　　者：陈升阳[1] 陈艳军[1] 胡水全 程冰冰[1] 仝昊 周百中[1] 李晓勇[1] CHEN Shengyang;CHEN Yanjun;HU Shuiquan;CHENG Bingbing;TONG Hao;ZHOU Baizhong;LI Xiaoyong(The Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第五附属医院,郑州450052

出　　处：《实用医学杂志》2021年第14期1795-1799,共5页The Journal of Practical Medicine

基　　金：河南省医学科技攻关计划联合共建项目(编号:LHGJ20200473)。

摘　　要：目的探讨长链非编码RNA(lncRNA)HOXA簇反义RNA2(HOXA⁃AS2)在胰腺癌组织中表达,以及沉默该基因对胰腺癌细胞增殖和侵袭力的影响。方法选取2015年8月至2020年8月在我院行根治性手术治疗的胰腺癌患者97例,培养人胰腺癌PANC⁃1和AsPC⁃1细胞并分别分为si⁃HOXA⁃AS2组、si⁃Con组和空白组,分别转染干扰序列、阴性对照序列和不作任何处理,使用RT⁃PCR检测胰腺癌和癌旁组织中HOXA⁃AS2表达,以及不同组细胞中HOXA⁃AS2表达,分别使用CCK⁃8和Transwell法检测细胞增殖活性及细胞侵袭力。结果HOXA⁃AS2在胰腺癌组织中表达量为(2.21±0.20),高于癌旁组织的(1.03±0.14),差异有统计学意义(t=48.030,P<0.001);胰腺癌组织中HOXA⁃AS2表达量在不同TNM分期、分化程度和是否发生淋巴结转移差异有统计学意义(P<0.05);PANC⁃1和AsPC⁃1细胞中,与si⁃Con组和空白组比较,si⁃HOXA⁃AS2组HOXA⁃AS2表达量明显降低(P<0.05),si⁃HOXA⁃AS2组24、48、72和96 h时吸光度OD值均低于si⁃Con组和空白组(P<0.05),si⁃HOXA⁃AS2组侵袭细胞数均低于si⁃Con组和空白组(P<0.05)。结论HOXA⁃AS2在胰腺癌组织中表达量升高,且与恶性进展临床指标相关,沉默其表达可减少细胞增殖、抑制细胞侵袭力。Objective To investigate the expression of lncRNA HOXA⁃AS2 in pancreatic cancer tissues,and the effect of silencing this gene on the proliferation and invasiveness of pancreatic cancer cells.Methods A total of 97 cases of patients with pancreatic cancer underwent radical surgery in our hospital were selected from August 2015 to August 2020.The human pancreatic cancer PANC⁃1 and AsPC⁃1 cells were cultured and were divide into si⁃HOXA⁃AS2 group,si⁃Con group and blank group,respectively,transfected with the interference sequence,negative control sequence and no treatment.RT⁃PCR was used to detect the expressions of HOXA⁃AS2 in pancreatic cancer and adjacent tissues,as well as the expression of HOXA⁃AS2 of cells in different groups.CCK⁃8 and Transwell methods were used to detect cell proliferation activity and cell invasiveness.Results The expression level of HOXA⁃AS2 in pancreatic cancer tissue was(2.21±0.20),which was higher than(1.03±0.14)in adjacent tissues,and the difference was statistically significant(t=48.030,P<0.001).The differences of the expres⁃sion levels of HOXA⁃AS2 in pancreatic cancer tissues between different TNM stages,degree of differentiation and whether lymph node metastasis were statistically significant(P<0.05).In PANC⁃1 and AsPC⁃1 cells,compared with the si⁃Con group and the blank group,the expression level of HOXA⁃AS2 in the si⁃HOXA⁃AS2 group was significantly decreased(P<0.05),and the absorbance OD values at 24,48,72 and 96 h in the si⁃HOXA⁃AS2 group were lower than those in the si⁃Con group and the blank group(P<0.05),and the number of invasive cells in the si⁃HOXA⁃AS2 group was lower than that in the si⁃Con group and the blank group(P<0.05).Conclusion The expression level of HOXA⁃AS2 is increased in pancreatic cancer tissues and in correspondance to clinical indicators of malignant progression.Silencing its expression can reduce cell proliferation and inhibit cell invasion.

关 键 词：胰腺癌 lncRNA HOXA⁃AS2 临床指标 细胞增殖 细胞侵袭 

分 类 号：R735.9[医药卫生—肿瘤]