Effect of ATM/CHK2/CDC25A Signal Pathway on the Resistance of Colorectal Cancer Cells to L-OHP  

作　　者：Xiaoli YAN Peng YAN Jing XUE Yanfang FAN Jining ZHENG 

机构地区：[1]Chengde Medical University, Chengde 067000, China

出　　处：《Medicinal Plant》2021年第3期69-71,共3页药用植物：英文版

基　　金：the Key Subjects(Pathology and Pathophysiology)at Colleges and Universities of Hebei Province;Natural Science Research Project of Chengde Medical University(201830);Science and Technology Research Project of Colleges and Universities in Hebei Province(ZD2020137).

摘　　要：[Objectives]To compare the expression of key factors ATM,CHK2,CDC25A and CDK2 in ATM/CHK2/CDC25A signal pathway in colorectal cancer cell line HCT116 and L-OHP-resistant cell line HCT116/L-OHP,and to explore the relationship between ATM/CHK2/CDC25A signal pathway and resistance of colorectal cancer cells to L-OHP.[Methods]RT-qPCR was used to detect the expression of ATM,CHK2,CDC25A and CDK2 mRNA in colorectal cancer cell line HCT116 and L-OHP-resistant cell line HCT116/L-OHP,and Western blot was used to detect the expression of corresponding proteins in these two cell lines.[Results]The expression of ATM,CHK2,CDC25A,CDK2 mRNA and protein in HCT116/L-OHP was significantly higher than that in HCT116.[Conclusions]ATM/CHK2/CDC25A signal pathway was associated with resistance of colorectal cancer cell line HCT116 to L-OHP.Blocking this signal pathway may partially reverse the chemotherapy resistance of colorectal cancer patients with L-OHP resistance.

关 键 词：Colorectal cancer ATM CHK2 CDC25A 

分 类 号：R73[医药卫生—肿瘤]