长链非编码RNA尿路上皮癌胚抗原1/微RNA-143-3p/成纤维细胞生长因子1轴对胃癌细胞BGC-823增殖、迁移及侵袭的影响  

Effect of long non-coding RNA urothelial carcinoma antigen 1/microRNA-143-3p/fibroblast growth factor 1 axis on proliferation,migration and invasion of gastric cancer BGC-823 cell

作　　者：段聿 陈吉[1] 崔宏[1] 高美丽[1] 赵翠娟[1] 牛昊书[1] 王举 DUAN Yu;CHEN Ji;CUI Hong;GAO Meili;ZHAO Cuijuan;NIU Haoshu;WANG Ju(Department of Gastroenterology,Inner Mongolia Baogang Hospital,Baotou 014010,Inner Mongolia,China;Department of Gastrointestinal Surgery,Inner Mongolia Autonomous Region People′s Hospital,Hohhot 010017,Inner Mongolia,China)

机构地区：[1]内蒙古包钢医院消化内科,内蒙古包头014010 [2]内蒙古自治区人民医院胃肠外科,内蒙古呼和浩特010017

出　　处：《新乡医学院学报》2021年第7期607-611,共5页Journal of Xinxiang Medical University

基　　金：国家自然科学基金资助项目(编号:81660415)。

摘　　要：目的探讨长链非编码RNA(lncRNA)尿路上皮癌胚抗原1(UCA1)/微RNA(miR)-143-3p/成纤维细胞生长因子1(FGF1)轴对胃癌细胞BGC-823增殖、迁移、侵袭的影响。方法取对数生长期的胃癌细胞BGC-823接种至24孔板,然后分为对照组、空载组和沉默组。对照组细胞不进行处理,空载组和沉默组细胞分别转染control-si、silncRNA UCA1序列。采用划痕实验和Transwell实验检测各组细胞增殖、迁移能力,实时荧光定量聚合酶链反应法检测各组细胞中lncRNA UCA1、miR-143-3p、FGF1基因表达情况。结果对照组与空载组细胞各时间点细胞增殖能力比较差异均无统计学意义(P>0.05);培养24、48、72 h时,沉默组细胞的增殖能力均显著低于对照组和空载组(P<0.05)。3组细胞的增殖能力均随时间的延长而升高,各时间点之间两两比较差异均有统计学意义(P<0.05)。沉默组细胞迁移率和侵袭能力均显著低于对照组和空载组(P<0.05);对照组与空载组细胞迁移率和侵袭能力比较差异无统计学意义(P>0.05)。沉默组细胞中lncRNA UCA1、FGF1基因相对表达量显著低于对照组和空载组,miR-143-3p基因相对表达量显著高于对照组和空载组(P<0.05);对照组与空载组细胞中lncRNA UCA1、miR-143-3p、FGF1基因相对表达量比较差异均无统计学意义(P>0.05)。结论lncRNA UCA1低表达可显著抑制胃癌细胞BGC-823的增殖、迁移和侵袭能力,可能是通过调控lncRNA UCA1/miR-143-3p/FGF1轴发挥作用。Objective To investigate the effect of long non-coding RNA(lncRNA)urothelial carcinoma antigen 1(UCA1)/microRNA(miR)-143-3p/fibroblast growth factor 1(FGF1)axis on proliferation,migration and invasion of gastric cancer BGC-823 cell.Methods The BGC-823 cells in logarithmic growth phase were inoculated into 24 well plates and then they were divided into the control group,empty group and silence group.The cells in control group were not treated,the cells in empty group and silence group were transformed with control-si,siRNA UCA1 sequence,respectively.The cell proliferation and migration ability were detected by scratch test and Transwell test.The expressions of lncRNA UCA1,miR-143-3p and FGF1 gene of cells in each group were detected by real-time fluorescent quantitative polymerase chain reaction.Results There was no significant difference in the cell proliferation ability between the control group and the empty group at each time point(P>0.05);the cell proliferation ability in the silence group was significantly lower than that in the control group and the empty group at 24,48 and 72 h after culture(P<0.05).The cell proliferation ability in the three groups increased with the prolong of time,and the differences between each time point were statistically significant(P<0.05).The cell migration rate and the number of transmembrane cells in the silence group were significantly lower than those in the control group and the empty group(P<0.05);there was no significant difference in cell migration rate and the number of transmembrane cells between the control group and the empty group(P>0.05).The relative expressions of lncRNA UCA1 and FGF1 gene of cells in the silence group were significantly lower than those in the control group and the empty group,and the relative expression of miR-143-3p of cells was significantly higher than that in the control group and the empty group(P<0.05);there was no significant difference in the relative expression of lncRNA UCA1,miR-143-3p and FGF1 gene of cells between the control group an

关 键 词：胃癌 长链非编码RNA 尿路上皮癌胚抗原1 增殖 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]