lncRNA UCA1通过激活miR-143/HK2通路促进皮肤鳞状细胞癌A431细胞增殖、侵袭和EMT  

LncRNA UCA1 stimulates proliferation, invasion and EMT of cutanous squamous cell carcinoma A431 cells through miR-143/HK2 axis

作　　者：张永红 李存涛 张玉红 姚丽 ZHANG Yonghong;LI Cuntao;ZHANG Yuhong;YAO Li(Department of Dermatology,Zhengzhou Central Hospital,Zhengzhou University,Zhengzhou 450000,China)

机构地区：[1]郑州大学附属郑州中心医院皮肤科,河南郑州450000

出　　处：《皮肤性病诊疗学杂志》2021年第3期174-181,共8页Journal of Diagnosis and Therapy on Dermato-venereology

基　　金：河南省医学科技攻关计划项目(2018020214)。

摘　　要：目的:探讨长链非编码RNA UCA1 (lncRNA UCA1)在皮肤鳞状细胞癌A431细胞中的表达及其对A431细胞增殖、侵袭及其上皮间质转化(EMT)的影响。方法:通过RT-qPCR检测皮肤鳞状细胞癌、癌旁正常组织、A431细胞、HaCaT细胞中lncRNA UCA1的表达;siRNA技术下调lncRNA UCA1表达,利用四甲基偶氮唑蓝(MTT)、细胞侵袭实验和Western blot检测下调lncRNA UCA1对A431细胞增殖、侵袭和EMT的影响。通过生物信息学miRanda软件和双荧光素酶报告基因实验分析lncRNA UCA1和微小RNA-143(miR-143)之间的关系。RT-qPCR检测miR-143在A431细胞、HaCaT细胞中的表达。同时上调lncRNA UCA1和miR-143表达,分析lncRNA UCA1通过miR-143对A431细胞增殖、侵袭和EMT的影响。通过生物信息学TargetScan软件和双荧光素酶报告基因实验检测miR-143与己糖激酶Ⅱ(HK2)之间的关系;RT-qPCR检测HK2在A431细胞、HaCaT细胞中的表达。siRNA技术下调HK2表达,分析A431细胞增殖、侵袭和EMT能力。结果:在A431细胞中lncRNA UCA1的表达上调(P<0.01),miR-143表达下调(P<0.01),HK2表达上调(P<0.01)。siRNA下调lncRNA UCA1表达明显抑制了A431细胞增殖、侵袭与EMT;lncRNA UCA1靶向且负调控miR-143;下调miR-143的表达促进A431细胞增殖、侵袭与EMT;上调lncRNA UCA1通过miR-143促进A431细胞增殖、侵袭与EMT。miR-143与HK2具有靶向关系。siRNA下调HK2表达抑制了A431细胞增殖、侵袭与EMT,lncRNA UCA1通过miR-143促进HK2表达。结论:lncRNA UCA1在皮肤鳞状细胞癌A431细胞中表达上调且通过miR-143/HK2轴促进A431细胞增殖、侵袭及其EMT。Objective:To investigate the expression levels of long noncoding RNA UCA1 in A431 cells and its effects on proliferation, invasion and epithelial-mesenchymal transition of A431 cells.Methods:The expression levels of lncRNA UCA1 in skin squamous cell carcinoma, paracancerous tissues, A431 cells and HaCaT cells were assessed by Quantitative Real-time PCR.Following down-regulation of lncRNA UCA1 with siRNA, proliferation, invasion and EMT of A431 cells were measured by methyl thiazolyl tetrazolium, Transwell and Western blot, respectively. The relationship between lncRNA UCA1 and microRNA-143 was analyzed by miRanda and double luciferase reporter gene assay. RT-qPCR was used to determine the expression levels of miR-143 in A431 cells and HaCaT cells. To determine whether the effects of lncRNA UCA1 is via miR-143, proliferation, invasion and EMT of A431 cells were analyzed following simultaneous upregulation of lncRNA UCA1 and miR-143. The relationship between miR-143 and Hexokinase II was analyzed by TargetScan and double luciferase reporter gene assay. RT-qPCR was used to detect HK2 expression in A431 cells and HaCaT cells. Proliferation, invasion and EMT of A431 cells were analyzed following down-regulation of HK2 with siRNA. Results:In A431 cells, the expression levels of both lncRNA UCA1 and HK2 were upregulated(P<0.01), while the expression levels of miR-143 were down-regulated(P<0.01). Down-regulation of lncRNA UCA1 significantly inhibited proliferation, invasion and EMT of A431 cells. lncRNA UCA1 negatively regulated miR-143 expression.Down-regulation of miR-143 stimulated proliferation, invasion and EMT of A431 cells. Upregulation of lncRNA UCA1 promoted proliferation, invasion and EMT of A431 cells through miR-143.Down-regulation of HK2 with siRNA inhibited A431 cell proliferation, invasion and EMT. lncRNA UCA1 upregulated HK2 expression through miR-143.Conclusions:Expression of lncRNA UCA1 is upregulated in cutaneous squamous cell carcinoma A431 cells. lncRNA UCA1 stimulates proliferation, invasion and EMT

关 键 词：lncRNA UCA1 MIR-143 HK2 A431细胞 增殖 EMT 

分 类 号：R739.5[医药卫生—肿瘤]