氨基酸树脂中Fmoc的含量测定  

Determination of Fmoc in Amino Acid Resin

作　　者：李宜航 LI Yi-hang(Changde Vocational Technical College,Hunan Changde 415000,China)

机构地区：[1]常德职业技术学院,湖南常德415000

出　　处：《广州化工》2021年第14期94-95,98,共3页GuangZhou Chemical Industry

摘　　要：Fmoc即9-芴甲氧羰基,保护多肽的的固相合成。在多肽合成的过程中,保护基Fmoc的去除不仅是必不可少的反应步骤,同时通过对去除的Fmoc含量的测定可以得出含有Fmoc氨基酸树脂的取代率,进而达到对多肽合成过程进行检测的目的。本方案利用紫外分光光度法对多肽合成过程中氨基酸保护基Fmoc的含量进行测定,可直观检测出出Fmoc氨基酸树脂中Fmoc的含量和装载量,从而对Fmoc保护多肽合成过程进行实时监控。Fmoc is the solid-phase synthesis of 9-fluorenylmethyloxycarbonyl,which protects peptides.In the process of peptide synthesis,the removal of the protective group Fmoc is not only an indispensable reaction step,at the same time,by measuring the content of the removed Fmoc,the substitution rate of the resin containing Fmoc can be obtained,thereby achieving the detection of the peptide synthesis process.Ultraviolet spectrophotometrywas used to determine the content of the amino acid protective group Fmoc during the peptide synthesis process,and can visually detect the content and loading of Fmoc inFmoc amino acid resin,thereby real-time monitoring of Fmoc protective peptide synthesis process.

关 键 词：多肽合成 Fmoc的含量测定 紫外分光光度法 

分 类 号：R914.5[医药卫生—药物化学]