Gene and protein expression profiles of olfactory ensheathing cells from olfactory bulb versus olfactory mucosa  

作　　者：Yuan-Xiang Lan Ping Yang Zhong Zeng Neeraj Yadav Li-Jian Zhang Li-Bin Wang He-Chun Xia 

机构地区：[1]School of Clinical Medicine,Ningxia Medical University,Yinchuan,Ningxia Hui Autonomous Region,China [2]Ningxia Human Stem Cell Institute,General Hospital of Ningxia Medical University,Yinchuan,Ningxia Hui Autonomous Region,China [3]Department of Neurosurgery,General Hospital of Ningxia Medical University,Yinchuan,Ningxia Hui Autonomous Region,China [4]Clinical Laboratory Center,General Hospital of Ningxia Medical University,Yinchuan,Ningxia Hui Autonomous Region,China [5]School of International Education,Ningxia Medical University,Yinchuan,Ningxia Hui Autonomous Region,China [6]Biochip Research Center,General Hospital of Ningxia Medical University,Yinchuan,Ningxia Hui Autonomous Region,China

出　　处：《Neural Regeneration Research》2022年第2期440-449,共10页中国神经再生研究（英文版）

基　　金：supported by Key Research Projects of Ningxia Hui Autonomous Region of China,No.2018BCG01002(to HCX);the Natural Science Foundation of Ningxia Hui Autonomous Region of China,No.NZ17150(to PY)。

摘　　要：Olfactory ensheathing cells(OECs) from the olfactory bulb(OB) and the olfactory mucosa(OM) have the capacity to repair nerve injury. However, the difference in the therapeutic effect between OB-derived OECs and OM-derived OECs remains unclear. In this study, we extracted OECs from OB and OM and compared the gene and protein expression profiles of the cells using transcriptomics and non-quantitative proteomics techniques. The results revealed that both OB-derived OECs and OM-derived OECs highly expressed genes and proteins that regulate cell growth, proliferation, apoptosis and vascular endothelial cell regeneration. The differentially expressed genes and proteins of OB-derived OECs play a key role in regulation of nerve regeneration and axon regeneration and extension, transmission of nerve impulses and response to axon injury. The differentially expressed genes and proteins of OM-derived OECs mainly participate in the positive regulation of inflammatory response, defense response, cytokine binding, cell migration and wound healing. These findings suggest that differentially expressed genes and proteins may explain why OB-derived OECs and OM-derived OECs exhibit different therapeutic roles. This study was approved by the Animal Ethics Committee of the General Hospital of Ningxia Medical University(approval No. 2017-073) on February 13, 2017.

关 键 词：biological process cellular component GENE Gene Ontology Kyoto Encyclopedia of Genes and Genomes molecular function olfactory bulb olfactory ensheathing cells olfactory mucosa PROTEIN 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]