低温胁迫下不同咖啡生理生化指标的变化及耐寒性评价  

Changes of Physiological,Biochemical and Evaluation of Cold Tole-rance in Coffea

作　　者：黄丽芳[1] 李金芹 龙宇宙[1] 董云萍[1] 王晓阳[1] 林兴军[1] 孙燕[1] 陈鹏[1] 闫林[1] HUANG Lifang;LI Jinqin;LONG Yuzhou;DONG Yunping;WANG Xiaoyang;LIN Xingjun;SUN Yan;CHEN Peng;YAN Lin(Spice and Beverage Research Institute,Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Genetic Re-sources Utilization of Spice and Beverage Crops,Ministry of Agriculture and Rural Affairs/Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops,Wanning,Hainan 571533,China;College of Tropical Crops,Yunnan Agricultural University,Pu’er,Yunnan 665099,China)

机构地区：[1]中国热带农业科学院香料饮料研究所/农业农村部香辛饮料作物遗传资源利用重点实验室/海南省热带香辛饮料作物遗传改良与品质调控重点实验室,海南万宁571533 [2]云南农业大学热带作物学院,云南普洱665099

出　　处：《热带作物学报》2021年第7期1941-1947,共7页Chinese Journal of Tropical Crops

基　　金：海南省自然科学基金项目(No.319QN318);中国热科院基本科研业务费项目(No.1630142017001);物种保护项目(No.18200037)。

摘　　要：以咖啡主栽品种大粒种、中粒种和小粒种咖啡为材料,进行8℃低温胁迫,研究其在胁迫条件下植株的伤害情况和生理生化指标变化,并利用隶属函数法对3种咖啡进行综合评价。结果表明:低温胁迫条件下,3种咖啡叶片受害程度差异显著,小粒种咖啡比大粒种和中粒种咖啡具有明显的耐寒表型;小粒种咖啡的可溶性糖和可溶性蛋白含量在胁迫72 h时,较大粒种和中粒种的高。3种咖啡的抗氧化酶变化趋势都是先升后降,大粒种和中粒种咖啡的抗氧化酶在各处理间都处于较低的水平,小粒种咖啡在胁迫48、72 h时仍表现活跃;咖啡的丙二醛和相对电导率的变化幅度与其抗寒性呈现负相关。通过隶属函数法综合评价,3种咖啡抗寒性由强到弱排序依次为:小粒种>大粒种>中粒种。Coffea liberica,Coffea canephora and Coffea arabica,treated with 8℃,were used to study the leaf physio-logical responses,and the cold tolerance of the cultivars was comprehensively evaluated using membership function.Under low temperature stress,C.arabica showed stronger cold-tolerance than C.liberica and C.canephora,and the damage degree of the leaves was significant.The content of soluble sugar and protein of C.arabica was higer than of that of C.liberica and C.canephora under 8℃for 72 h.The antioxidant enzymes of the cultivars increased first and then decreased.The antioxidant enzymes of C.liberica and C.canephora were in a low level among all treatments,while that of C.arabica was still active under 48 and 72 h stress.Malondialdehyde and relative electrical conductivity was negatively correlated with cold resistance.The Evaluation on the cold tolerance of the cultivars was in the order C.arabica>C.liberica>C.canephora.

关 键 词：咖啡 抗寒性 生理生化 隶属函数法 

分 类 号：S571.2[农业科学—作物学]