Two-Photon Luminescence Imaging of Bacillus Spores Using Peptide-Functionalized Gold Nanorods  

作　　者：Wei He Walter A.Henne Qingshan Wei Yan Zhao Derek D.Doorneweerd Ji-Xin Cheng Philip S.Low Alexander Wei 

机构地区：[1]Purdue University,Department of Chemistry,560 Oval Drive,West Lafayette,IN 47907,USA [2]Purdue University,Weldon School of Biomedical Engineering,206 S.Martin Jischke Drive,West Lafayette,IN 47907,USA [3]Center of Sensing Science and Technology,Purdue University,West Lafayette,IN 47907,USA

出　　处：《Nano Research》2008年第6期450-456,共7页纳米研究（英文版）

基　　金：This work is supported by the National Institute of Health(EB-001777);also by the Department of Defense(W911SR-08-C-0001);administered through the U.S.Army RDECOM(Edgewood Contracting Division)and the Center for Sensing Science and Technology at Purdue University。

摘　　要：Bacillus subtilis spores(a simulant of Bacillus anthracis)have been imaged by two-photon luminescence(TPL)microscopy,using gold nanorods(GNRs)functionalized with a cysteine-terminated homing peptide.Control experiments using a peptide with a scrambled amino acid sequence confi rmed that the GNR targeting was highly selective for the spore surfaces.The high sensitivity of TPL combined with the high affi nity of the peptide labels enables spores to be detected with high fi delity using GNRs at femtomolar concentrations.It was also determined that GNRs are capable of signifi cant TPL output even when irradiated at near infrared(NIR)wavelengths far from their longitudinal plasmon resonance(LPR),permitting considerable fl exibility in the choice of GNR aspect ratio or excitation wavelength for TPL imaging.

关 键 词：BIONANOTECHNOLOGY NANORODS nonlinear optics pathogen detection PEPTIDES 

分 类 号：TB3[一般工业技术—材料科学与工程]