Pullulan Acetate Coated Magnetite Nanoparticles for Hyper-Thermia:Preparation,Characterization and In Vitro Experiments  

作　　者：Fuping Gao Yuanyuan Cai Jing Zhou Xiaoxue Xie Weiwei Ouyang Yuhui Zhang Xufei Wang Xiaodong Zhang Xiaowen Wang Lingyun Zhao Jintian Tang 

机构地区：[1]Key Laboratory of Particle&Radiation Imaging,Ministry of Education,Institute of Medical Physics and Engineering,Department of Engineering Physics,Tsinghua University,Beijing 100084,China [2]Department of Biopharmaceutics,Beijing University of Traditional Chinese Medicine,Beijing 100102,China [3]Department of Oncology,Xiangya Hospital of Central South University,Changsha 410008,China

出　　处：《Nano Research》2010年第1期23-31,共9页纳米研究（英文版）

基　　金：This study was supported by the Beijing Municipal Science&Technology Commission(No.Z07000200540704);the China Postdoctoral Science Foundation(No.20090450417)and the Tsinghua Yuyuan Medical Fund.

摘　　要：Amphipathic polymer pullulan acetate (PA)-coated magnetic nanoparticles were prepared and characterized by various physicochemical means. The cytotoxicity and cellular uptake of the magnetic nanoparticles were examined. The hyperthermic effect of the magnetic nanoparticles on tumor cells was evaluated. Transmission electron microscopy (TEM) showed that the PA coated magnetic nanoparticles (PAMNs) had spherical morphology. Dynamic light scattering (DLS) showed that the size distribution of PAMNs was unimodal,with an average diameter of 25.8 nm ± 6.1 nm. The presence of the adsorbed layer of PA on the magnetite surface was confirmed by Fourier transform infrared (FTIR) spectroscopy. Magnetic measurements revealed that the saturation magnetization of the PAMNs reached 51.9 emu/g and the nanoparticles were superparamagnetic. Thermogravimetric analysis (TGA) showed that the Fe_(3)O_(4) particles constituted 75 wt% of the PAMNs. The PAMNs had good heating properties in an alternating magnetic field. Cytotoxicity assay showed that PAMNs exhibited no significant cytotoxicity against L929 cells. TEM results showed that a large number of PAMNs were internalized into KB cells. PAMNs have good hyperthermia effect on KB cells in vitro by magnetic field induced hyperthermia. These novel magnetic nanoparticles have great potential as magnetic hyperthermia mediators.

关 键 词：Magnetic nanoparticles pullulan acetate HYPERTHERMIA CYTOTOXICITY cellular uptake 

分 类 号：TB3[一般工业技术—材料科学与工程]