基于转录组测序的枣黑顶病发病机理初探  

A Preliminary Study on Pathogenesis of Jujube Black Tip Disease Based on RNA-seq

作　　者：陈欣[1] 邓彩萍[1] 牛雅琪 王升级 贾举庆[2] CHEN Xin;DENG Caiping;NIU Yaqi;WANG Shengji;JIA Juqing(College of Forestry,Shanxi Agricultural University, Taigu Shanxi 030801,China;College of Agriculture, Shanxi Agricultural University, Taigu Shanxi 030801,China)

机构地区：[1]山西农业大学林学院,山西太谷030801 [2]山西农业大学农学院,山西太谷030801

出　　处：《西北农业学报》2021年第6期907-913,共7页Acta Agriculturae Boreali-occidentalis Sinica

基　　金：山西省应用基础研究项目(201701D121006)。

摘　　要：为进一步诠释枣黑顶病发病机理,本研究使用Illumina高通量测序技术对自然发病枣果(ZJINF)、氢氧化钙处理枣果(ZJPRE)及健康枣果(ZJCON)进行分析,拟从转录组水平探明枣果黑顶病发生的分子机理。基因功能注释(GO)分析结果表明,ZJINF vs ZJCON中的差异表达基因主要体现在钙离子结合、钙依赖磷脂结合、金属离子体内平衡、防御反应等生物学功能上,导致枣果对外界不良环境的抗性减弱。代谢通路(KEGG)富集分析显示:植物-病原体互作、内质网蛋白质加工、吞噬体等代谢通路富集程度较高。ZJINF vs ZJCON中,CALR、CRT被抑制低表达;而喷施氢氧化钙之后,枣果的CALR、CRT两种钙蛋白表达水平均与健康枣果无显著差异。表明CALR、CRT在黑顶病的抗病过程中可能有着重要的作用。Jujube black tip disease seriously reduces the economic value of jujube fruit and restricts the development of jujube industry,but its pathogenesis is still unclear.In order to explore the molecular mechanism of jujube black tip disease,Illumina high-throughput sequencing was used to analyze the transcriptome of jujube fruits which were infected naturally black tip disease(ZJINF)and treated with calcium hydroxide(ZJPRE)as well as healthy jujube fruits(ZJCON).GO analysis showed that the deferentially expressed genes between ZJINF and ZJCON were mainly enriched in the biological functions of calcium ion binding,calcium-dependent phospholipid binding,metal ion homeostasis and defense response,suggesting reduced resistance of jujube fruit to adverse environment.KEGG analysis showed that plant-pathogen interaction,end oplasmic reticulum protein processing,phagocytes and other metabolic pathways were specifically enriched.The expression of CALR and CRT was inhibited in ZJINF vs ZJCON.However,there was no significant difference between healthy jujube fruits and fruits sprayed with calcium hydroxide.The results showed that CALR and CRT may play an important role in the resistance to black tip disease,and this study will provide theoretical basic for further interpretation of the pathogenesis of jujube black tip disease.

关 键 词：壶瓶枣 转录组测序 黑顶病 发病机理 差异表达基因 

分 类 号：S665.1[农业科学—果树学]