急性胰腺炎患者血清cf-DNA/NETs、MAP1-LC3、DPB表达水平及临床意义  

The levels of serum cf⁃DNA/NETs,MAP1⁃LC3,DPB in patients with acute pancreatitis and their clinical significance

作　　者：袁燕文 陈孟君[1] 许哲[1] 黄妙兴[1] 陈至操 叶衬轩 YUAN Yanwen;CHEN Mengjun;XU Zhe;HUANG Miaoxing;CHEN Zhicao;YE Chenxuan(Department of Gastroenterology,Dongguan People􀆳s Hospital,Dongguan,Guangdong,China,523000)

机构地区：[1]东莞市人民医院消化内科,广东东莞523000

出　　处：《分子诊断与治疗杂志》2021年第7期1055-1058,1062,共5页Journal of Molecular Diagnostics and Therapy

基　　金：东莞市科技计划项目(2015105101195)。

摘　　要：目的探究血清游离DNA/中性粒细胞胞外诱捕网(cf-DNA/NETs)、微管相关蛋白1轻链3(MAP1-LC3)、维生素D结合蛋白(DPB)在急性胰腺炎患者中的表达水平及临床意义。方法收集2015年9月至2020年9月本院收治的急性胰腺炎患者150例,随访30 d,根据预后分为生存组(138例)与死亡组(12例),比较两组临床资料、治疗前后血清cf-DNA/NETs、MAP1-LC3、DPB水平、急性生理与慢性健康评价系统Ⅱ(APACHEⅡ)评分、Ranson评分,分析血清cf-DNA/NETs、MAP1-LC3、DPB与APACHEⅡ、Ranson评分相关性及血清各指标与急性胰腺炎患者预后的关系。结果死亡组入院时、治疗3、7 d后血清cf-DNA/NETs、MAP1-LC3高于生存组,DPB低于生存组,差异均有统计学意义(P<0.05);死亡组入院时、治疗3、7 d后APACHEⅡ、Ranson评分高于生存组,差异有统计学意义(P<0.05)。入院时、治疗3、7 d后血清cf-DNA/NETs、MAP1-LC3与APACHEⅡ、Ranson评分呈正相关,DPB与APACHEⅡ、Ranson评分呈负相关(P<0.05);入院时、治疗3、7 d后血清cf-DNA/NETs、MAP1-LC3、DPB均为急性胰腺炎患者预后的影响因素(P<0.05)。结论急性胰腺炎患者血清cf-DNA/NETs、MAP1-LC3、DPB表达水平明显异常,且与患者APACHEⅡ、Ranson评分及预后密切相关。Objective To explore the expression levels and clinical significance of serum cell⁃free DNA/nertrophil extracellular traps(cf⁃DNA/NETs),microtubule⁃associated protein 1 light chain 3(MAP1⁃LC3),vitamin D binding protein(DPB)in patients with acute pancreatitis.Methods From September 2015 to September 2020,150 cases of acute pancreatitis in our hospital were collected.Followed up for 30 days,according to the prognosis,they were divided into the survival group(138 cases)and the death group(12 cases).The clinical data,serum cf⁃DNA/NETs,MAP1⁃LC3,DPB levels,acute physiology,and chronic health evaluationⅡ(APACHEⅡ)score,Ranson score before and after treatment were compared between the two groups.The correlation between serum cf⁃DNA/NETs,MAP1⁃LC3,DPB and APACHEⅡ,Ranson score and the relationship between serum indicators and the prognosis of patients with acute pancreatitis were analyzed.Results Serum cf⁃DNA/NETs and MAP1⁃LC3 in the death group were higher than those in the survival group at admission,3 and 7 days after treatment,and DPB was lower than that in the survival group.The difference was statistically significant(P<0.05).The APACHEⅡand Ranson scores of the death group were higher than those of the survival group at admission,3 and 7 days after treatment,and the difference was statistically significant(P<0.05).Serum cf⁃DNA/NETs and MAP1⁃LC3 were positively correlated with APACHEⅡand Ranson scores at admission,3 and 7 days after treatment.DPB was negatively correlated with APACHEⅡand Ranson scores(P<0.05).Serum cf⁃DNA/NETs,MAP1⁃LC3 and DPB at admission,3 and 7 days after treatment were all influencing factors for the prognosis of patients with acute pancreatitis(P<0.05).Conclusion The expression levels of serum cf⁃DNA/NETs,MAP1⁃LC3 and DPB in patients with acute pancreatitis are abnormal,and are closely related to the patients􀆳APACHEⅡ,Ranson score and prognosis.

关 键 词：急性胰腺炎 游离DNA/中性粒细胞胞外诱捕网 微管相关蛋白1轻链3 维生素D结合蛋白 

分 类 号：R576[医药卫生—消化系统]