Transdermal delivery of fluorescein isothiocyanate-dextrans using the combination of microneedles and low-frequency sonophoresis  

作　　者：Boonnada Pamornpathomkul Sureewan Duangjit Suvida Laohapatarapant Theerasak Rojanarata Praneet Opanasopit Tanasait Ngawhirunpat 

机构地区：[1]Faculty of Pharmacy,Silpakorn University,Nakhon Pathom 73000,Thailand [2]Pharmaceutical Development of Green Innovations Group(PDGIG),Faculty of Pharmacy,Silpakorn University,Nakhon Pathom 73000,Thailand [3]Faculty of Pharmaceutical Sciences,Ubon Ratchathani University,Ubon Ratchathani 34190,Thailand

出　　处：《Asian Journal of Pharmaceutical Sciences》2015年第5期415-424,共10页亚洲药物制剂科学（英文）

基　　金：the Thailand Research Fund through the Basic Research Grant(Grant No.5680016);the Faculty of Pharmacy,Silpakorn University,and Mr.Subhachai Saibour,the factory director and department manager at Bangkok Lab and Cosmetics Co.,Ltd.,for facilities and financial support.

摘　　要：This study aimed to evaluate the patient-friendly methods that are used in the delivery of hydrophilic macromolecules into deep skin layers,in particular,the combination of microneedles patch(MNs patch)and low-frequency sonophoresis(SN).The hydrophilic macromolecule drug fluorescein isothiocyanate(FITC)-dextrans(FD-4:MW 4.4 kDa)was used as the model drug in our experimental design.In this study,excised porcine skin was used to investigate and optimize the key parameters that determine effective MNs-and SNfacilitated FD-4 delivery.In vitro skin permeation experiments revealed that the combination of MNs patch with SN had a superior enhancing effect of skin permeation for FD-4 compared to MNs alone,SN alone or untreated skin,respectively.The optimal parameters for the combination of MNs and SN included the following:10 N insertion force of MNs,4 W/cm^(2)SN intensity,6 mm radiation diameter of the SN probe,2 min application time,and the continuous mode duty cycle of SN.In addition,vertical sections of skin,clearly observed under a confocal microscope,confirmed that the combination of MNs and SN enhanced permeation of FD-4 into the deep skin layers.These studies suggest that the combination of MNs and SN techniques could have great potential in the delivery of hydrophilic macromolecules into deep skin.

关 键 词：MICRONEEDLE SONOPHORESIS Fluorescein isothiocyanate-dextran Transdermal drug delivery 

分 类 号：TG1[金属学及工艺—金属学]