HPLC–MS and HPLC–MS/MS analysis of seven active constituents of Xiao-Xu-Ming decoction and application to a pharmacokinetic study after oral administration to rat  

作　　者：Yilin Wang Chunguang Ding Caisheng Wu Kehe Du Jinlan Zhangn Hailin Qin Jinfeng Hou Guanhua Du 

机构地区：[1]State Key Laboratory of Bioactive Substances and Functions of Natural Medicines,Institute of Materia Medica,Chinese Academy of Medical Sciences and Peking Union Medical College,Beijing 100050,China

出　　处：《Acta Pharmaceutica Sinica B》2012年第2期188-197,共10页药学学报（英文版）

基　　金：the National Natural Science Foundation of China(No.30630073);the Innovation Method Fund from Ministry of Science and Technology of China(2009IM031600)for financial support.

摘　　要：Xiao-xu-ming decoction(XXMD)is a traditional Chinese medicine that has been widely used to treat theoplegia and its sequelae.This paper reports the development of three separate assays based on reversed phase high-performance liquid chromatography–mass spectrometry(HPLC–MS)and HPLC–MS/MS for the determination of seven active constituents of XXMD viz oroxylin A-7-O-glucuronide,wogonoside,liquiritigenin,cimifugin,5-O-methylvisammiol,glycyrrhizic acid and glycyrrhetinic acid in rat plasma.All calibration curves were linear(r >0.99)with lower limits of quantitation(LLOQs)<12.4 ng/mL.Intra-and inter-day precisions(as relative standard deviation)were all <10.7% with recoveries in the range of 88.7–113%.In addition,the seven analytes were shown to be stable in rat plasma samples under relevant storage conditions.The validated methods were successfully applied to a pharmacokinetic study in rat after oral administration of XXMD.

关 键 词：Xiao-Xu-Ming decoction HPLC-MS HPLC-MS/MS Pharmacokinetics Oroxylin A-7-O-glucuronide WOGONOSIDE LIQUIRITIGENIN Cimifugin 5-O-methylvisammiol Glycyrrhizic acid Glycyrrhetinic acid 

分 类 号：R28[医药卫生—中药学]