磷霉素壳寡糖盐的合成与抗菌活性测试  

Synthesis and Antibacterial Activity of Fosfomycin Chitosan Oligosaccharide Salt

作　　者：李爽 李丽[1] 姜志炜 金学平 余磊[2,3] 傅曦卉 王艳武[1] 李雪 祝宏 LI Shuang;LI Li;JIANG Zhiwei;JIN Xueping;YU Lei;FU Xihui;WANG Yanwu;LI Xue;ZHU Hong(School of Chemical Engineering and Pharmacy,School of Environmental Ecology and Biological Engineering,Wuhan Institute of Technology,Wuhan 430205,China;Wuhan Drug Solubilization and Delivery Technology Research Center,Wuhan 430205,China;Wuhan Vocational College of Software and Engineering,Wuhan430205,China)

机构地区：[1]武汉工程大学化工与制药学院,环境生态与生物工程学院,湖北武汉430205 [2]武汉市药物增溶工程技术研究中心,湖北武汉430205 [3]武汉软件工程职业学院,湖北武汉430205

出　　处：《武汉工程大学学报》2021年第4期372-375,397,共5页Journal of Wuhan Institute of Technology

基　　金：武汉工程大学研究生教育创新基金(CX2019031);武汉工程大学校长基金(XZJJ2020021,XZJJ2020022)。

摘　　要：为了丰富磷霉素类药物的种类并获得更好的抗菌效果,采用拼合的方法合成了磷霉素壳寡糖盐。通过红外光谱、核磁共振氢谱对其结构初步地进行了结构表征,并采用滤纸片法验证和比较了磷霉素钙和磷霉素壳寡糖盐对大肠杆菌、金黄色葡萄球菌、肺炎克雷伯菌、铜绿假单胞菌的抑菌作用。以生物活性测试中抑菌圈的大小为评价指标,设计均匀实验,比较了左磷右胺盐与壳寡糖的质量比、反应温度、反应时间对磷霉素壳寡糖盐的抑菌活性的影响。确定了磷霉素壳寡糖盐的最佳制备条件是:左磷右胺盐与壳寡糖的质量比为1∶3,反应温度为25℃,反应时间为0.50 h。To enrich the types of fosfomycin drugs and obtain better antibacterial activity,the fosfomycin chitosan oligosaccharide salt was synthesized by a splicing method.Its structure was initially characterized by infrared spectroscopy and 1H nuclear magnetic resonance spectroscopy.Its antibacterial activities towards escherichia coli,staphylococcus aureus,klebsiella pneumoniae and pseudomonas aeruginosa were further verified by the filter paper method and compared with that of fosfomycin calcium.Taking the size of inhibition zone in biological activity test as the evaluation index,we used uniform design of tests to compare the effects of the mass ratio of levophosphatidylamine salt and chitooligosaccharide,reaction temperature and reaction time on the antibacterial activity of fosfomycin chitosan oligosaccharide salt.The optimum preparation conditions are determined as follows:the mass ratio of levophosphoramine salt to chitosan oligosaccharide is 1∶3,the reaction temperature is 25℃ and the reaction time is 0.50 h.

关 键 词：磷霉素 壳寡糖 抑菌活性 

分 类 号：O626[理学—有机化学]