鹅去氧胆酸对雄激素非依赖性前列腺癌细胞增殖的影响及机制研究  

Effect of CDCA on the proliferation of androgen independent prostate cancer cells and mechanism research

作　　者：刘念[1] 赵军[1] 滕昊林 周洪澜[1] 傅耀文[1] LIU Nian;ZHAO Jun;TENG Hao-lin(Department of Urology,First Hospital of Jilin University,Jilin Changchun 130021,China)

机构地区：[1]吉林大学第一医院泌尿外科,吉林长春130021

出　　处：《中国实验诊断学》2021年第7期1078-1081,共4页Chinese Journal of Laboratory Diagnosis

基　　金：吉林省卫生计生委卫生服务能力提升项目资助(2018SCZWSZX-056)。

摘　　要：目的探讨鹅去氧胆酸(CDCA)对雄激素非依赖性前列腺癌细胞生物学特性的影响及可能机制。方法CDCA作用于雄激素非依赖前列腺癌细胞DU145后,通过油红染色检测细胞内脂肪含量变化,观察CDCA对前列腺癌细胞内脂类的影响;采用CCK8方法测定细胞增殖,采用Real-time PCR及Western blot检测细胞脂类代谢关键因子FASN及ACC的表达。结果CDCA能够降低DU145细胞内脂质水平,对正常前列腺细胞RWPE-1无明显作用。CDCA作用后,DU145增殖水平明显下降,且随着作用时间延长,抑制作用更显著。Real-time PCR结果显示CDCA能够抑制FASN及ACC基因的表达水平,同时FASN的蛋白表达水平及ACC磷酸化水平在CDCA作用后均有不同程度的降低。结论CDCA能够影响DU145细胞增殖,同时对细胞内脂类的合成有明显的抑制作用,其效应机制可能与调节脂类合成酶的表达有关。Objective To explore the effect of CDCA on the biological characteristics of androgen independent prostate cancer cells and possible mechanism.Methods Androgen independent prostate cancer cell line DU145 were treated with CDCA.Lipid accumulation in cells were observed by Oil red O.The cell proliferation was analyzed by CCK8.The mRNA and protein levels of FASN and ACC which are the key enzymes in lipid metabolism were determined by Realtime PCR and Western-blot.Results CDCA treatment could reduce lipid content in DU145cells but not in RWPE-1 cells.Cell proliferation was significantly decreased after CDCA treatment,and the inhibitory effect was more obvious with the extended time of treatment.Real-time PCR showed the mRNA levels of FASN and ACC were reduced,and the protein levels of FASN and the phosphorylation level of ACC had been inhibited after CDCA treatment.Conclusion CDCA can inhibit DU145 cells proliferation and reduced de novo lipid synthesis.The role of CDCA may be involve in regulating lipogenic enzymes expression.

关 键 词：鹅去氧胆酸 前列腺癌 脂质 细胞增殖 

分 类 号：R737.25[医药卫生—肿瘤]