Noninvasive Ultrasound Deep Brain Stimulation for the Treatment of Parkinson’s Disease Model Mouse  

作　　者：Hui Zhou Lili Niu Long Meng Zhengrong Lin Junjie Zou Xiangxiang Xia Xiaowei Huang Wei Zhou Tianyuan Bian Hairong Zheng 

机构地区：[1]Paul C.Lauterbur Research Center for Biomedical Imaging,Institute of Biomedical and Health Engineering,Shenzhen Institutes of Advanced Technology,Chinese Academy of Sciences,China [2]Shenzhen College of Advanced Technology,University of Chinese Academy of Sciences,China

出　　处：《Research》2019年第1期782-794,共13页研究（英文）

基　　金：We wish to thank Dr.Jun Jia(Capital Medical University)for assisting us with experimental design and Dr.Yunhui Liu(Shenzhen Institutes of Advanced Technology)for technical guidance.This work was supported by the National Natural Science Foundation of China(Grants nos.81527901,11534013,11774371,11574341,11674347,and 11874381);Natural Science Foundation of Guangdong Province(2017B030306011);Youth Innovation Promotion Association CAS(2018393).

摘　　要：Modulating basal ganglia circuitry is of great signifcance in the improvement of motor function in Parkinson’s disease(PD).Here,for the frst time,we demonstrate that noninvasive ultrasound deep brain stimulation(UDBS)of the subthalamic nucleus(STN)or the globus pallidus(GP)improves motor behavior in a subacute mouse model of PD induced by 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(MPTP).Immunohistochemical c-Fos protein expression confrms that there is a relatively high level of c-Fos expression in the STN-UDBS and GP-UDBS group compared with sham group(both p<0.05).Furthermore,STN-UDBS or GPUDBS signifcantly increases the latency to fall in the rotarod test on day 9(p<0.05)and decreases the time spent climbing down a vertical rod in the pole test on day 12(p<0.05).Moreover,our results reveal that STN-UDBS or GP-UDBS protects the dopamine(DA)neurons from MPTP neurotoxicity by downregulating Bax(p<0.001),upregulating Bcl-2(p<0.01),blocking cytochrome c(Cyt C)release from mitochondria(p<0.05),and reducing cleaved-caspase 3 activity(p<0.01)in the ipsilateral substantia nigra(SN).Additionally,the safety of ultrasound stimulation is characterized by hematoxylin and eosin(HE)and Nissl staining;no hemorrhage or tissue damage is detected.Tese data demonstrate that UDBS enables modulation of STN or GP neural activity and leads to neuroprotection in PD mice,potentially serving as a noninvasive strategy for the clinical treatment of PD.

关 键 词：STIMULATION DEEP enable 

分 类 号：R74[医药卫生—神经病学与精神病学]