异养硝化-好氧反硝化菌株的分离筛选及复配菌剂对河水的净化效果  

Isolation of heterotrophic nitrification-aerobic denitrification bacteria strains and purification of river water by mixed cultures

作　　者：汤默然 李茹莹[1] TANG Moran;LI Ruying(School of Environment Science and Engineering,Tianjin University,Tianjin 300350)

机构地区：[1]天津大学环境科学与工程学院,天津300350

出　　处：《环境科学学报》2021年第7期2657-2663,共7页Acta Scientiae Circumstantiae

基　　金：国家水体污染控制与治理科技重大专项(No.2017ZX07106004)。

摘　　要：从天然河水中富集分离出8株异养硝化-好氧反硝化(HN-AD)菌株.将单菌株根据其自身的种属类别及脱氮性能复配成5种由不同菌株构成的菌剂,优选出脱氮效果最佳的复配菌剂-2,其包括6株菌株,分别为Pseudomonas stutzeri MR1,Pseudomonas sp. MR^(2),Pseudomonas sp. MR3,Pseudomonas balearica MR4,Klebsiella variicola MR6和Catellibacterium terrae MR8.将复配菌剂-2投加至COD_(Cr)/TN比分别为20和5的河水中,其对NO^(-)_(3)-N的去除率分别为87.1%和97.5%,期间无NO^(-)_(2)-N积累;对NH^(+)_(4)-N去除效果在第1 d分别达到96.6%和57.6%.复配菌剂可以获得对河水较高的反硝化脱氮效率,并可以强化河水中NH^(+)_(4)-N的去除,对实际河水的脱氮净化具有较强的应用潜能.In this study,eight strains of heterotrophic nitrification-aerobic denitrification(HN-AD)strains were isolated from river water and then five mixed cultures were prepared according to the nitrogen removal performance and the classification of each strain.The mixed culture-2 performed better nitrogen removal capacity than others,which consisted of six strains of HN-AD bacteria including Pseudomonas stutzeri MR1,Pseudomonas sp.MR2,Pseudomonas sp.MR3,Pseudomonas balearica MR4,Klebsiella variicola MR6 and Catellibacterium terrae MR8.The mixed culture-2 was inoculated into the raw river water with CODCr/TN ratios of 20 and 5 respectively,achieving NO^(-)_(3)-N removal efficiencies of 87.1%and 97.5%,respectively,without NO^(-)_(2)-N accumulation.The NH+4-N removal efficiencies reached 96.6% and 57.6%,respectively,at the first day.The HN-AD mixed culture-2 could obtain high denitrification efficiency,and could enhance the NH^(+)_(4)-N removal,suggesting a potential for the nitrogen removal of practical river water.

关 键 词：异养硝化-好氧反硝化菌 复配菌剂 河水 脱氮 

分 类 号：X172[环境科学与工程—环境科学] X522