Quantitative determination of isradipine in dog plasma by an ultra performance liquid chromatographyetandem mass spectrometry method  

作　　者：Xiaojing Yang Haiyan Xu Jingjing Gan Li Li Jiayang Li Yi Jin Bo Yuan 

机构地区：[1]Shenyang Pharmaceutical University,No.103,Wenhua Road,Shenyang 110016,China

出　　处：《Asian Journal of Pharmaceutical Sciences》2013年第5期312-317,共6页亚洲药物制剂科学（英文）

摘　　要：A simple,sensitive and specific ultra performance liquid chromatographyetandem mass spectrometry(UPLCeMS/MS)method was developed for the analysis of isradipine in dog plasma.After extracted with organic solvent,dog plasma samples were analyzed on an Acquity UPLC BEH C18 column interfaced with a triple quadrupole tandem mass spectrometer in positive electrospray ionization mode.Acetonitrile:water:formic acid(80:20:0.3,v/v/v)was used as the mobile phase at a flow rate of 0.2 ml/min.The chromatographic run time of each sample was 1.4 min.The calibration curve in plasma was linear in the range of 0.1e40.0 ng/ml.The intra-and inter-day precision was within 13.5%in terms of relative standard deviation(RSD%)and the accuracy was required to be 96.5%e98.4%.The validated UPLCeMS/MS method was successfully applied in a pharmacokinetic study of controlledrelease isradipine in dogs.

关 键 词：ISRADIPINE UPLCeMS/MS PHARMACOKINETICS PLASMA 

分 类 号：R28[医药卫生—中药学]