叶酸偶联量子点免疫脂质磁珠法检测卵巢上皮性癌循环肿瘤细胞的效果及循环肿瘤细胞与患者临床病理特征的关系  

Efficacy of folate-coupled quantum dots immunomagnetic beads method for detecting circulating tumor cells in epithelial ovarian cancer and the association of circulating tumor cells with clinicopathological features

作　　者：韩晓芳[1] 尹海珍 张荣[3] 刘建荣[1] Han Xiaofang;Yin Haizhen;Zhang Rong;Liu Jianrong(Department of Reproductive Medicine,Shanxi Provincial People's Hospital,Taiyuan 030012,China;Central Laboratory,Shanxi Provincial People's Hospital,Taiyuan 030012,China;Department of Obstetrics and Gynecology,Shanxi Provincial People's Hospital,Taiyuan 030012,China)

机构地区：[1]山西省人民医院生殖医学科,太原030012 [2]山西省人民医院中心实验室,太原030012 [3]山西省人民医院妇产科,太原030012

出　　处：《肿瘤研究与临床》2021年第6期440-444,共5页Cancer Research and Clinic

基　　金：山西省卫生计划生育委员会科研课题联合项目(2018028);山西省科技厅面上项目(201901D111443)。

摘　　要：目的:探讨叶酸偶联量子点(FA-QD)免疫脂质磁珠法检测卵巢上皮性癌循环肿瘤细胞(CTC)的有效性及CTC与患者临床病理特征的相关性。方法:选取2019年8月至2020年1月在山西省人民医院就诊的卵巢上皮性癌患者67例。将卵巢癌SKOV-3细胞分为5个细胞数梯度(0、100、150、200、300个),比较上皮细胞黏附分子(EpCAM)免疫磁珠法(单标法)和FA-QD免疫脂质磁珠法(双标法)对CTC的检出率。应用FA-QD免疫脂质磁珠法检测卵巢癌患者外周血中CTC数量,荧光显微镜下见阳性CTC者即为CTC阳性患者,分析CTC与患者临床病理特征及肿瘤标志物的关系。结果:FA-QD免疫脂质磁珠法对SKOV-3细胞CTC的平均捕获率高于EpCAM免疫磁珠法(83.4%比70.3%)。在67例卵巢癌患者中,Ⅰ~Ⅱ期患者CTC阳性者比例为30.0%(3/10),Ⅲ期为91.9%(34/37),Ⅳ期为95.0%(19/20),差异有统计学意义(P<0.05);淋巴结转移患者CTC阳性者比例为97.1%(33/34),高于淋巴结未转移者的69.7%(23/33),差异有统计学意义(P<0.05);人附睾蛋白4(HE4)>110 pmol/L患者CTC阳性者比例为58.8%(10/17),低于HE4≤110 pmol/L患者的92.0%(46/50),差异有统计学意义(P=0.005);而年龄、绝经情况、病理分化程度、远处转移、癌胚抗原125(CA125)、癌胚抗原199(CA199)、血清癌胚抗原(CEA)分层患者间CTC阳性患者比例差异均无统计学意义(均P>0.05)。结论:FA-QD免疫脂质磁珠法可有效检测卵巢上皮性癌患者外周血CTC。卵巢上皮性癌患者CTC水平与淋巴结转移、临床TNM分期及HE4水平有关。Objective To explore the effectiveness of folate-coupled quantum dots(FA-QD)immunomagnetic beads method for detecting circulating tumor cells(CTC)in epithelial ovarian cancer and the association of CTC with clinicopathological features of tumor patients.Methods A total of 67 ovarian cancer patients in Shanxi Provincial People's Hospital from August 2019 to January 2020 were selected.Ovarian cancer SKOV-3 cells were divided into 5 cell number gradients(0,100,150,200,300),the detection rates of CTC were compared by using epithelial cell adhesion molecule(EpCAM)immunomagnetic beads(single standard method)and FA-QD immunomagnetic beads method(double standard method).The number of CTC in peripheral blood of ovarian cancer patients was detected by using FA-QD immunomagnetic beads method,and those with positive CTC under fluorescence microscope were treated as CTC positive patients.The association of CTC with clinicopathological factors and tumor markers of tumor patients was analyzed.Results The average capture efficiency rate of CTC in SKOV-3 cells detected by FA-QD immunomagnetic beads method(83.4%)was higher than that by EpCAM immunomagnetic beads method(70.3%).Among 67 patients of ovarian cancer,the proportion of CTC positive patients was 30.0%(3/10)in stageⅠ-Ⅱ,91.9%(34/37)in stageⅢ,95.0%(19/20)in stageⅣ,and the difference was statistically significant(P<0.05).The proportion of CTC positive patients with lymph node metastasis was higher than that of patients without lymph node metastasis[97.1%(33/34)vs.69.7%(23/33)],and the difference was statistically significant(P<0.05).The proportion of CTC positive patients with human epididymis protein 4(HE4)>110 pmol/L was lower than that of patients with HE4≤110 pmol/L[58.8%(10/17)vs.92.0%(46/50)],and the difference was statistically significant(P=0.005).There were no statistically significant differences in the proportion of CTC positive patients stratified by age,menopause,pathological differentiation,distant metastasis,carbohydrate antigen(CA)125,CA199,carcino-e

关 键 词：卵巢肿瘤 肿瘤细胞 循环 免疫磁化分离 量子点 

分 类 号：R737.31[医药卫生—肿瘤]