白细胞介素11促进Dami细胞增殖、分化及与抗凋亡基因的关系  

Interleukin-11 promotes the proliferation and differentiation of Dami cells and its relationship with anti-apoptosis genes

作　　者：龙轶 张培先[1] 石岚 邓磊[1] 丁万宝 卢久琴 LONG Yi;ZHANG Peixian;SHI Lan;DENG Lei;DING Wanbao;LU Jiuqin(Oncology Department,Yan'an Hospital Affiliated to Kunming Medical University,Yunnan Kunming 650051,China.)

机构地区：[1]昆明医科大学附属延安医院肿瘤科,云南昆明650051

出　　处：《现代肿瘤医学》2021年第16期2787-2791,共5页Journal of Modern Oncology

摘　　要：目的:探讨白细胞介素11(IL-11)对巨核细胞系Dami增殖、分化及抗凋亡基因的作用特点及其机制。方法:利用1640培养基对Dami细胞进行培养,用25~1600 ng/mL浓度IL-11和Dami细胞共同孵育24 h后,离心取上清沉淀即为生成的血小板,用自动血细胞分析仪检测血小板的数量;MTT比色法观察不同浓度IL-11对Dami细胞的增殖抑制效应;流式细胞仪检测不同浓度IL-11作用Dami细胞后细胞周期及生成的血小板表面CD61阳性比例;Western blot检测不同浓度IL-11作用Dami细胞后抗凋亡基因Bcl-xl、Survivin的表达。结果:浓度为25~1600 ng/mL的IL-11处理Dami细胞24 h后,均可以观察到血小板明显增加,但浓度大于200 ng/mL后,血小板生成达到峰值平台;同时发现100 ng/mL、400 ng/mL和1600 ng/mL刺激Dami细胞24 h后,CD61阳性血小板的比例分别为35.3%、36.1%和51.6%,虽然在浓度大于200 ng/mL后,IL-11刺激Dami细胞生成血小板的数量不会明显随着剂量增加而增加,但是随着浓度提高,CD61阳性比例却明显提高;随着IL-11浓度的增加,Dami细胞的增殖呈现剂量、时间依赖效应(P<0.01);100~1600 ng/mL浓度的IL-11均可以降低G 0/G 1期细胞的比例,而S期细胞比例明显上升,同时抗凋亡基因Bcl-xl和Survivin表达上调。结论:IL-11可以促进巨核细胞系Dami增殖及分化,其可能是通过上调抗凋亡基因Bcl-xl、Survivin表达促进血小板的生成。Objective:To investigate the effect of interleukin-11(IL-11)on the proliferation and differentiation of megakaryocyte line Dami and the mechanism of anti-apoptosis genes.Methods:The Dami cells were cultured in 1640 medium and incubated with IL-11 at a concentration of 25~1600 ng/mL for 24 hours.The supernatant was centrifuged and precipitated to form platelets.The number of platelets was detected by automatic hematology analyzer.MTT colorimetric assay was used to observe the inhibitory effect of IL-11 at different concentrations on Dami cells.Flow cytometry was used to detect the cell cycle of Dami cells affected by IL-11 at different concentrations and the proportion of CD61 positive on the platelet surface.Western blot analysed Bcl-xl and Survivin expression in Dami cells treated with IL-11 at different concentrations.Results:After 24 hours of treatment with IL-11 at a concentration of 25~1600 ng/mL,significant increases in platelets could be observed,but platelet production reached a peak platform when the concentration was greater than 200 ng/mL.After 100 ng/mL,400 ng/mL and 1600 ng/mL to stimulate Dami cell about 24 hours,CD61 positive percentages of platelets were 35.3%,36.1%and 51.6%,while in concentration greater than 200 ng/mL,IL-11 stimulating Dami cell to generate the number of platelets will not obviously increase as the dose increased,but with the higher concentration,CD61 positive ratio was improved obviously.With the increase in IL-11 concentration,the proliferation of Dami cells presented a dose-dependent and time-dependent effect(P<0.01).IL-11 concentration of 100~1600 ng/mL can reduce the proportion of G 0/G 1 phase cells,while the proportion of S phase cells was significantly increased,and the expression of anti-apoptotic genes Bcl-xl and Survivin was up-regulated.Conclusion:IL-11 can promote the proliferation and differentiation of megakaryocyte line Dami,possibly by up-regulating anti-apoptotic genes Bcl-xl and Survivin expression to promote platelet production.

关 键 词：血小板减少 白细胞介素11 巨核细胞 细胞凋亡 

分 类 号：R730.5[医药卫生—肿瘤]