牵牛子多糖PNP-5的结构表征及体外生物活性研究  

Structure Characterization and in Vitro Bioactivities of Polysaccharide PNP-5 from Pharbitis nil

作　　者：孙延平[1,2] 王博譞[3] 刘艳 梁军[1] 刘艳鑫[1] 曹琦 杨炳友[1] 王秋红[4] 匡海学 SUN Yanping;WANG Boxuan;LIU Yan;LIANG Jun;LIU Yanxin;CAO Qi;YANG Bingyou;WANG Qiuhong;KUANG Haixue(Key Laboratory of Chinese Materia Medica,Ministry of Ed cation,Heilongjiang Key Laboratory of Traditional Chinese Medicine and Natural Medicine Pharmacodynamic Material Bases,Heilongjiang University of Chinese Medicine,Harbin 150040,China;Laboratory of Pharmaceutical Resource Discovery,alian Institute of Chemical Physics,Chinese Academy of Scien es,Dalian 116023,China;Harbin Medical University,Harbin 150081,China;College of Chinese Materia Medica,Guangdong Pharmaceutical University,Guangzhou 512000,China)

机构地区：[1]黑龙江中医药大学教育部北药基础与应用研究重点实验室,黑龙江省中药及天然药物药效物质基础研究重点实验室,黑龙江哈尔滨150040 [2]中国科学院大连化学物理研究所药用资源开发实验室,辽宁大连116023 [3]哈尔滨医科大学,黑龙江哈尔滨150081 [4]广东药科大学中药学院,广东广州512000

出　　处：《中医药学报》2021年第8期42-48,共7页Acta Chinese Medicine and Pharmacology

基　　金：国家自然科学基金项目(81603282);中国博士后基金项目(2015M581373);黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2016206);黑龙江中医药大学“优秀青年教师支持计划”(2018RCQ05)。

摘　　要：目的:从牵牛子中分离纯化牵牛子多糖PNP-5,并对其进行结构表征及体外生物活性研究。方法:采用UV、IR、HPSEC、HPLC、GC-MS、高碘酸氧化Smith降解、甲基化及13C-NMR等方法对PNP-5结构进行表征,并采用体外清除ABTS自由基活性及对体外小鼠脾细胞增殖活性影响考察体外生物活性。结果:PNP-5相对分子质量为1.78×106Da,PNP-5主链可能以Rha为核心连接少量的Glc、Gal和Man作为主链,支链部分主要包含GlcA、GalA。这些醛酸可能是在Rha的C-4位、Gal的C-6位、Man的C-6位有连接的分支点。PNP-5的末端残基可能为α-Araf-(1→和β-D-Xylp-(1→。体外生物活性研究结果表明,PNP-5具有明显的体外ABTS+·清除能力和体外抑制小鼠脾细胞增殖活性。结论:PNP-5为单糖组成丰富支链较多结构复杂的酸性杂多糖,具有一定体外生物活性。Objective:To isolate and purify polysaccharide-5(PNP-5)from Pharbitis nil,and to study the structural characterization and bioactivities in vitro.Methods:The structure of PNP-5 was characterized by UV,IR,HPSEC,HPLC,GC-MS,periodate oxidation and Smith degradation,methylation analysis and 13C-NMR.ABTS radical scavenging capacity and its effect on proliferation of spleen cells were measured in vitro.Results:The molecular weight of PNP-5 was estimated to be 1.78×106 Da.The main chain of PNP-5 was composed of Rha with less of Glc,Gal and Man,and the branched chain mainly contained GlcA and GalA,which might have joined branch points at the C-4 of Rha,C-6 of Gal and C-6 of Man.The terminal residues of PNP-5 could beα-Araf-(1→andβ-D-Xylp-(1→.The in vitro activity test showed that PNP-5 exhibited strong ABTS free radical scavenging ability and could significantly inhibit the proliferation of spleen cells in mice.Conclusion:PNP-5 is a complex acidic heteropolysaccharides with abundant branched chains composed of multiple monosaccharides and has certain bioactivity in vitro.

关 键 词：牵牛子 多糖 结构表征 自由基清除 脾细胞增殖 

分 类 号：R284[医药卫生—中药学]