Cluster size distribution of cancer cells in blood using stopped-flow centrifugation along scale-matched gaps of a radially inclined rail  

作　　者：Macdara Glynn Charles Nwankire Kate Lemass David J.Kinahan Jens Ducrée 

机构地区：[1]Biomedical Diagnostics Institute,National Centre for Sensor Research,School of Physical Sciences,Dublin City University,Ireland

出　　处：《Microsystems & Nanoengineering》2015年第1期97-105,共9页微系统与纳米工程（英文）

基　　金：This work was supported in part by Enterprise Ireland(Grant No.CF 20111317);Science Foundation Ireland(Grant No.10/CE/B1821).

摘　　要：There is increasing evidence that,in addition to their presence,the propensity of circulating tumour cells to form multi-cellular clusters bears significant information about both cellular resistance to chemotherapy and overall prognosis.We present a novel two-stage,stopped-flow,continuous centrifugal sedimentation strategy to measure the size distributions of events(defined here as cells or clusters thereof)in a blood sample.After off-chip removal of red blood cells,healthy white blood cells are sequestered by negative-immunocapture.The purified events are then resolved along a radially inclined rail featuring a series of gaps with increasing width,each connected to a designated outer collection bin.The isolation of candidate events independent of targetspecific epitopes is successfully demonstrated for HL60(EpCAM positive)and sk-mel28(EpCAM negative)cells using identical protocols and reagents.The propensity to form clusters was quantified for a number of cell lines,showing a negligible,moderate or elevated tendency towards cluster formation.We show that the occupancy distribution of the collection bins closely correlates with the range of cluster sizes intrinsic to the specific cell line.

关 键 词：centrifugal microfluidics lab-on-a-disc cancer diagnostics cellular clustering 

分 类 号：R73[医药卫生—肿瘤]