肝硬化门静脉高压动物模型的实验对比研究  

A comparison between two animal models of liver cirrhosis and portal hypertension

作　　者：胡豆豆[1] 任万雷 仇莎 姜相君[1] HU Dou-dou;REN Wan-lei;QIU Sha;JIANG Xiang-jun(Department of Gastroenterology,Qingdao Municipal Hospital 266011;Department of Traditional Chinese Medicine,Qingdao Central Hospital 266042)

机构地区：[1]青岛市市立医院消化内二科,山东266011 [2]青岛大学附属第二医院青岛市中心医疗集团中医一科

出　　处：《肝脏》2021年第7期732-736,共5页Chinese Hepatology

基　　金：国家自然基金青年基金项目(81800543)。

摘　　要：目的对四氯化碳(CCl_(4))化学损伤性以及胆管结扎(BDL)机械梗阻性大鼠门静脉高压模型进行比较,对两种动物模型在门静脉高压形成过程中的特点进行探讨。方法CCl_(4)模型分为模型组和对照组,BDL模型分为模型组和假手术组。分别于造模后4周、8周、12周测量门静脉压力、取肝脾组织标本。从大鼠一般情况、门静脉压力数值、大体标本变化、病理形态学、体质量、肝脏湿重、脾脏湿重这几方面对两种动物模型进行比较分析。正态分布的资料以均数±标准差表示,两组比较采用t检验。结果CCl_(4)组肝脏大体变化以肝叶变形、粘连为主,病理表现为纤维间隔分割肝实质,假小叶形成。而BDL组肝叶形态尚存,肝脏呈深褐色,病理表现为大量胆管增生。就门脉高压形成过程而言,CCl_(4)组门静脉压力呈逐渐升高趋势,与对照组相比,造模8周、12周有统计学差异(P<0.05)。BDL组门静脉压力呈现短期迅速升高趋势,造模4周、8周及12周较假手术组均明显升高且有统计学差异(P<0.05)。在体质量、肝脏湿重、脾脏湿重这几个指标中,脾脏湿重与门脉压力升高趋势一致。在CCl_(4)组,脾脏质量亦是逐渐增加的。而在BDL组,造模4周脾脏质量较假手术组明显增加,造模8周、12周时较假手术组有统计学差异(P<0.05)。结论两种动物模型门静脉高压形成过程及压力变化趋势不同,本文为深入认识不同原因肝硬化门静脉高压提供了实验依据。Objective To establish and evaluate two rat models of portal hypertension induced by carbon tetrachloride(CCl_(4))and bile duct ligation(BDL).Methods 12 rats were randomly divided into CCl_(4)-induced liver injured group and a control group(N=6 in each group).Another 12 rats were divided into a BDL model group and a sham operation group(N=6 in each group).Portal pressures were measured and the liver and spleen tissue samples were taken at 4w,8w,and 12w after the treatments.Rats in the model groups were compared with relative control groups and analyzed for general conditions,portal pressure,gross specimen changes,pathological morphologies,body mass,liver wet weight,and spleen wet weight.Normal distributed data are expressed as“mean±standard deviation”,and t test is used for the comparison between two groups.Results The major changes of livers in the CCl_(4) model group were liver lobular deformation and adhesion.Fibrous septa and pseudolobules were observed as the pathological manifestation of liver parenchyma.In BDL model group,the shape of the liver lobes was still preserved,the livers were dark brown,and bile duct hyperplasia was the major pathological manifestation.In terms of the formation of portal hypertension,the portal pressure of rats in CCl_(4) model group showed a gradual increase and significantly higher than that of the control group at 8w and 12w of CCl_(4) treatment(P<0.05).The portal pressure of rats in BDL model group showed a short-term rapid increase,and significantly higher than those of the sham operation group at 4w,8w,and 12w after BDL(P<0.05).Among the body mass,liver wet weight,and spleen wet weight,the increase of spleen wet weight is in consistent with that of portal pressure.The spleen weight of the rats of CCl_(4) model group also gradually increased.The spleen weight of rats in the BDL model group was significantly higher than that of the sham operation group at 4w,and statistically different from those in the sham operation group at 8w and 12w after BDL(P<0.05).Conclusion Ther

关 键 词：门脉高压 肝硬化动物模型 胆管结扎 四氯化碳 

分 类 号：R57[医药卫生—消化系统]