A reporter for noninvasively monitoring gene expression and plant transformation  

作　　者：Yubing He Tao Zhang Hui Sun Huadong Zhan Yunde Zhao 

机构地区：[1]State Key Laboratory of Crop Genetics and Germplasm Enhancement,Nanjing Agricultural University,Nanjing 210095,China [2]National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan),Huazhong Agricultural University,Wuhan 430070,China [3]Section of Cell and Developmental Biology,University of California San Diego,9500 Gilman Drive,La Jolla,CA 92093-0116,USA [4]Tata Institute for Genetics and Society-UCSD,La Jolla,CA 92093-0335,USA

出　　处：《Horticulture Research》2020年第1期821-826,共6页园艺研究（英文）

基　　金：supported by grants from the National Transgenic Science and Technology Program(2019ZX08010-003,2019ZX08010-001)to Y.H.and H.Z.T.Z.is a TIGS postdoctoral fellow.W。

摘　　要：Reporters have been widely used to visualize gene expression,protein localization,and other cellular activities,but the commonly used reporters require special equipment,expensive chemicals,or invasive treatments.Here,we construct a new reporter RUBY that converts tyrosine to vividly red betalain,which is clearly visible to naked eyes without the need of using special equipment or chemical treatments.We show that RUBY can be used to noninvasively monitor gene expression in plants.Furthermore,we show that RUBY is an effective selection marker for transformation events in both rice and Arabidopsis.The new reporter will be especially useful for monitoring cellular activities in large crop plants such as a fruit tree under field conditions and for observing transformation and gene expression in tissue culture under sterile conditions.

关 键 词：TRANSFORMATION REPORTER STERILE 

分 类 号：Q94[生物学—植物学]