VcFT-induced mobile florigenic signals in transgenic and transgrafted blueberries  

作　　者：Guo-qing Song Aaron Walworth Tianyi Lin Qiuxia Chen Xiumei Han LIrina Zaharia Gan-yuan Zhong 

机构地区：[1]Plant Biotechnology Resource and Outreach Center,Department of Horticulture,Michigan State University,East Lansing,MI 48824,USA [2]Aquatic and Crop Resource Development,National Research Council of Canada,Saskatoon SK S7N 0W9,Canada [3]Grape Genetics Research Unit,USDA-ARS,Geneva,NY 14456,USA

出　　处：《Horticulture Research》2019年第1期407-421,共15页园艺研究（英文）

摘　　要：FLOWERING LOCUS T(FT)can promote early flowering in annual species,but such role has not been well demonstrated in woody species.We produced self and reciprocal grafts involving non-transgenic blueberry(NT)and transgenic blueberry(T)carrying a 35S-driven blueberry FT(VcFT-OX).We demonstrated that the transgenic VcFT-OX rootstock promoted flowering of non-transgenic blueberry scions in the NT(scion):T(rootstock)grafts.We further analyzed RNASeq profiles and six groups of phytohormones in both NT:T and NT:NT plants.We observed content changes of several hormone metabolites,in a descending order,in the transgenic NT:T,non-transgenic NT:T,and non-transgenic NT:NT leaves.By comparing differential expression transcripts(DETs)of these tissues in relative to their control,we found that the non-transgenic NT:T leaves had many DETs shared with the transgenic NT:T leaves,but very few with the transgenic NT:T roots.Interestingly,a number of these shared DETs belong to hormone pathway genes,concurring with the content changes of hormone metabolites in both transgenic and non-transgenic leaves of the NT:T plants.These results suggest that phytohormones induced by VcFT-OX in the transgenic leaves might serve as part of the signals that resulted in early flowering in both transgenic plants and the non-transgenic NT:T scions.

关 键 词：BLUE grafted ROOTS 

分 类 号：R28[医药卫生—中药学]