Identification of genes preferentially expressed in wild strawberry receptacle fruit and demonstration of their promoter activities  

作　　者：Rachel Shahan Dongdong Li Zhongchi Liu 

机构地区：[1]Department of Cell Biology and Molecular Genetics,University of Maryland,College Park,MD 20742,USA [2]Zhejiang University,College of Biosystems Engineering and Food Science,Key Laboratory of Agro-Products Postharvest Handling Ministry of Agriculture,Zhejiang Key Laboratory for Agri-Food Processing,Zhejiang University,Hangzhou 310058,People’s Republic of China [3]Department of Biology and Howar

出　　处：《Horticulture Research》2019年第1期1005-1014,共10页园艺研究（英文）

基　　金：supported by NSF grants(MCB0923913 and IOS1444987)to Z.L.

摘　　要：Fragaria vesca(F.vesca),the wild strawberry,is a diploid model for the commercial,octoploid strawberry as well as other members of the economically relevant Rosaceae family.Unlike the fruits of tomato and Arabidopsis,the fleshy fruit of strawberry is unique in that it is derived from the floral receptacle and has an external seed configuration.Thus,identification and subsequent characterization of receptacle-expressed genes may shed light on novel developmental processes or provide insight into how developmental regulation differs between receptacle-derived and ovary-derived fruits.Further,since fruit and flower tissues are the last organs to form on a plant,the development of receptacle fruitspecific promoters may provide useful molecular tools for research and application.In this work,we mined previously generated RNA-Seq datasets and identified 589 genes preferentially expressed in the strawberry receptacle versus all other profiled tissues.Promoters of a select subset of the 589 genes were isolated and their activities tested using a GUS transcriptional reporter.These promoters may now be used by the F.vesca research community for a variety of purposes,including driving expression of tissue-specific reporters,RNAi constructs,or specific genes to manipulate fruit development.Further,identified genes with receptacle-specific expression patterns,including MADS-Box and KNOX family transcription factors,are potential key regulators of fleshy fruit development and attractive candidates for functional characterization.

关 键 词：STRAW ATTRACTIVE FRUIT 

分 类 号：S66[农业科学—果树学]