Transcriptome of Erysiphe necator-infected Vitispseudoreticulata leaves provides insight into grapevineresistance to powdery mildew  

作　　者：Kai Weng Zhi-Qian Li Rui-Qi Liu Lan Wang Yue-Jin Wang Yan Xu 

机构地区：[1]State Key Laboratory of Crop Stress Biology in Arid Areas(Northwest A&F University),Yangling,shanxi 712100,China [2]College of Horticulture,Northwest A&F University,Yangling,shanxi 712100,China [3]Key Laboratory of Horticultural Plant Biology and Germplasm Innovation in Northwest China,Ministry of Agriculture,Yangling,shanxi 712100,China

出　　处：《Horticulture Research》2014年第1期86-97,共12页园艺研究（英文）

基　　金：This research was supported by the‘948’Program,Ministry of Agriculture,China(Grant No.2011-G21),by the Program for Young Talents in Northwest A&F University(NCET-10-0692,QN2011052);by a grant from the Program for Innovative Research Team of Grape Germplasm Resource and Breeding(2013KCT-25)to Yan Xu。

摘　　要：Powdery mildew(PM),which is caused by the pathogen Erysiphe necator(Schw.)Burr.,is the single most damaging disease of cultivated grapes(Vitis vinifera)worldwide.However,little is known about the transcriptional response of grapes to infection with PM.RNA-seq analysis was used for deep sequencing of the leaf transcriptome to study PM resistance in Chinese wild grapes(V.pseudoreticulata Baihe 35-1)to better understand the interaction between host and pathogen.Greater than 100 million(M)90-nt cDNA reads were sequenced from a cDNA library derived from PM-infected leaves.Among the sequences obtained,6541 genes were differentially expressed(DEG)and were annotated with Gene Ontology terms and by pathway enrichment.The significant categories that were identified included the following:defense,salicylic acid(SA)and jasmonic acid(JA)responses;systemic acquired resistance(SAR);hypersensitive response;plant–pathogen interaction;flavonoid biosynthesis;and plant hormone signal transduction.Various putative secretory proteins were identified,indicating potential defense responses to PMinfection.In all,318 putative R-genes and 183 putative secreted proteins were identified,including the defense-related R-genes BAK1,MRH1 and MLO3 and the defense-related secreted proteins GLP and PR5.The expression patterns of 16 genes were further illuminated by RT-qPCR.The present study identified several candidate genes and pathways that may contribute to PM resistance in grapes and illustrated that RNA-seq is a powerful tool for studying gene expression.The RT-qPCR results reveal that effective resistance responses of grapes to PM include enhancement of JA and SAR responses and accumulation of phytoalexins.

关 键 词：resistance VITIS interaction 

分 类 号：P45[天文地球—大气科学及气象学]