hsa_circ_0062019 promotes the proliferation,migration,and invasion of prostate cancer cells via the miR-195-5p/HMGA2 axis  

作　　者：Peiyu Wang Ligang Zhang Shuiping Yin Yuchen Xu Sheng Tai Li Zhang Chaozhao Liang 

机构地区：[1]Department of Urology,The First Affiliated Hospital of Anhui Medical University,Hefei 230022,China [2]Institute of Urology,Anhui Medical University,Hefei 230032,China [3]Anhui Province Key Laboratory of Genitourinary Diseases,Anhui Medical University,Hefei 230032,China

出　　处：《Acta Biochimica et Biophysica Sinica》2021年第7期815-822,共8页生物化学与生物物理学报（英文版）

基　　金：This work was supported by the grants from the National Natural Science Foundation of China(Nos.81870519,81700662,81630019,and 81470986);Scientific Research Foundation of the Institute for Translational Medicine of Anhui Province(No.2017ZHYX02);the Natural Science Research Project Funding of Higher Education Institutions of Anhui Province(No.KJ2019A0279);Cultivation Project of Young Top-Notch Talent Support from Anhui Medical University(AHMU);the Funding for Distinguished Young Scientists of the First Affiliated Hospital of AHMU.

摘　　要：Circular RNA(circRNA)is a new class of non-coding RNA.It was reported that circRNA involves in the metastasis of cancer.The aim of this study is to explore the role and mechanism of circRNA hsa_circ_0062019 in the development of prostate cancer(PCa).Our results showed that hsa_circ_0062019 was highly expressed in PCa cell lines.Cell Counting Kit-8 assay revealed that upregulation of hsa_circ_0062019 boosted PCa cell proliferation,and silencing of hsa_circ_0062019 inhibited cell proliferation.Meanwhile,transwell assay proved that upregulation of hsa_circ_0062019 facilitated PCa cell invasion and migration,while downregulation of hsa_circ_0062019 inhibited these malignant phenotypes.Furthermore,luciferase reporter assay proved the binding of hsa_circ_0062019 with miR-195-5p and the binding between miR-195-5p and high mobility group AT-hook 2(HMGA2),suggesting that hsa_circ_0062019 promoted the expression of HMGA2 by sponging miR-195-5p.In addition,our results revealed that the hsa_circ_0062019-induced PCa cell malignant phenotypes were notably reversed by the downregulation of HMGA2.Overall,our study demonstrated that hsa_circ_0062019 promoted PCa cell proliferation,migration,and invasion via upregulation of HMGA2 expression by sponging miR-195-5p.Our study proved a novel molecular mechanism of PCa development and provided a potential target for the treatment of PCa.

关 键 词：prostate cancer hsa_circ_0062019 competing endogenous RNA miR-195-5p high mobility group AT-hook 2 

分 类 号：R737.25[医药卫生—肿瘤]