Bromodomain-containing proteins BRD1, BRD2, and BRD13 are core subunits of SWI/SNF complexes and vital for their genomic targeting in Arabidopsis  

作　　者：Yaoguang Yu Wei Fu Jianqu Xu Yawen Lei Xin Song Zhenwei Liang Tao Zhu Yuhui Liang Yuanhao Hao Liangbing Yuan Chenlong Li 

机构地区：[1]State Key Laboratory of Biocontrol,Guangdong Provincial Key Laboratory of Plant Resources,School of Life Sciences,Sun Yat-Sen University,Guangzhou,China

出　　处：《Molecular Plant》2021年第6期888-904,共17页分子植物（英文版）

基　　金：supported by the National Natural Science Foundation of China to C.L.(32000380 and 31870289);Guangdong Basic and Applied Basic Research Foundation to C.L.(2021A1515011286);the Fundamental Research Funds for the Central Universities,Sun Yat-sen University to C.L(18lgzd12).

摘　　要：Switch defective/sucrose non-fermentable(SWI/SNF)chromatin remodeling complexes are multi-subunit machines that play vital roles in the regulation of chromatin structure and gene expression.However,the mechanisms by which SWI/SNF complexes recognize their target loci in plants are not fully understood.Here,we show that the Arabidopsis thaliana bromodomain-containing proteins BRD1,BRD2,and BRD13 are core subunits of SWI/SNF complexes and critical for SWI/SNF genomic targeting.These three BRDs interact directly with multiple SWI/SNF subunits,including the BRAHMA(BRM)catalytic subunit.Phenotypic and transcriptomic analyses of the brd1 brd2 brd13 triple mutant revealed that these BRDs act largely redundantly to control gene expression and developmental processes that are also regulated by BRM.Genome-wide occupancy profiling demonstrated that these three BRDs extensively colocalize with BRM on chromatin.Simultaneous loss of function of three BRD genes results in reduced BRM protein levels and decreased occupancy of BRM on chromatin across the genome.Furthermore,we demonstrated that the bromodomains of BRDs are essential for genomic targeting of the BRD subunits of SWI/SNF complexes to their target sites.Collectively,these results demonstrate that BRD1,BRD2,and BRD13 are core subunits of SWI/SNF complexes and reveal their biological roles in facilitating genomic targeting of BRM-containing SWI/SNF complexes in plants.

关 键 词：SWI/SNF complexes chromatin remodeling BROMODOMAIN BRAHMA ARABIDOPSIS 

分 类 号：Q943.2[生物学—植物学]