信阳毛尖茶水提物缓解PM_(2.5)致大鼠肺损伤的研究  

A Study of Xinyang Maojian Tea Water Extracts on Relieving Lung Injury Caused by PM_(2.5)

作　　者：刘建军[1,2,3] 李美凤 张静 田丽丽[1,2,4] 邓燕莉 林海燕[1,2] 黄建安 刘仲华[1,2] LIU Jianjun;LI Meifeng;ZHANG Jing;TIAN Lili;DENG Yanli;LIN Haiyan;HUANG Jian′an;LIU Zhonghua(Key Lab of Tea Science of Ministry of Education,Hunan Agricultural University,Changsha 410128,China;National Research Center of Engineering&Technology for Utilization of Functional Ingredients from Botanicals,Changsha 410128,China;Tea College,Guizhou University,Guiyang 550025,China;Shandong Institute of Pomology,Taian 271000,China)

机构地区：[1]湖南农业大学茶学教育部重点实验室,湖南长沙410128 [2]国家植物功能成分利用工程技术研究中心,湖南长沙410128 [3]贵州大学茶学院,贵州贵阳550025 [4]山东省果树研究所,山东泰安271000

出　　处：《茶叶科学》2021年第4期525-534,共10页Journal of Tea Science

基　　金：国家茶叶产业技术体系(CAR-23-11B);河南省高等学校重点科研项目(16A210051);国家自然科学基金(32060701)。

摘　　要：以健康雄性SD大鼠为试验对象,信阳毛尖茶水提物为受试物,气管滴注PM_(2.5)(Fine particulate matter)悬浮液建立慢性肺损伤模型,研究信阳毛尖茶水提物对滴注PM2.5大鼠体重增长率、肺组织形态学、肺组织病理学的影响,并对大鼠血清和支气管肺泡灌洗液(BALF)的生化指标进行测定。结果表明,信阳毛尖茶水提物一定程度上具有拮抗PM_(2.5)导致的大鼠体重增长率降低以及肺部损伤等作用;此外,信阳毛尖茶水提物可有效阻止大鼠血清和BALF中白细胞介素-1β(IL-1β)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)、免疫球蛋白G(IgG)含量和乳酸脱氢酶(LDH)活性上升,以及超氧化物歧化酶(SOD)活性的下降,其效果与浓度呈良好的剂量关系。In this study,the healthy male sprague-dawley(SD)rats were taken as experimental subjects,and the water extracts of Xinyang Maojian tea were used as test substances,while intratracheal instillation of PM2.5 suspension was established as chronic pulmonary injury model.Rats serum and biochemical indexes of bronchoalveolar lavage fluid(BALF)were tested.The results show that the tea extracts had antagonism effects on the reduce of rats growth rate and the lung injury caused by PM2.5.Furthermore,the tea water extracts could effectively lower the increase of IL-1β,IL-6,TNF-α,IgG and the activity of LDH,and keep the activity of SOD in rats serum and BALF,showing a dose-dependent effects.

关 键 词：信阳毛尖茶 缓解 PM_(2.5) 气管滴注 肺损伤 

分 类 号：S571[农业科学—作物学] R563[医药卫生—呼吸系统]