2,4-二硝基甲苯磺酸盐降解菌株的分离、鉴定及降解特性研究  

Isolation and Identification of a 2,4-Dinitrotosylate Degrading Strain and Its Degradation Characteristics

作　　者：徐文杰 赵泉林[2] 罗明汉 叶正芳[2] XU Wenjie;ZHAO Quanlin;LUO Minghan;YE Zhengfang(School of Environmental Engineering,Nanjing Institute of Technology,Nanjing 211167;College of Environmental Sciences and Engineering,Peking University,Beijing 100871)

机构地区：[1]南京工程学院环境工程学院,南京211167 [2]北京大学环境科学与工程学院,北京100871

出　　处：《北京大学学报（自然科学版）》2021年第4期733-738,共6页Acta Scientiarum Naturalium Universitatis Pekinensis

基　　金：南京工程学院校级科研基金(YKJ2019101);国家基础产品创新科研项目(15);北京市科技计划项目(Z151100000915065);江苏省高等学校自然科学研究面上项目(18KJB61006)资助。

摘　　要：针对TNT红水污染土壤生物修复菌种资源匮乏问题,从复合菌群中分离纯化出一株2,4-二硝基甲苯磺酸盐(DNTS)降解菌株X2,经生理生化分析及16S rDNA鉴定,该菌株属于鞘氨醇单胞菌属。进一步研究环境因素对菌株X2生长的影响,并探究X2对2,4-二硝基甲苯-3-磺酸盐(2,4-DNT-3-SO_(3)^(−))和2,4-二硝基甲苯-5-磺酸盐(2,4-DNT-5-SO_(3)^(−))的降解机理及对多种硝基芳香族炸药的降解效果。结果表明,X2的最佳生长条件为30℃,pH=7和1%盐度。菌株X2对初始浓度为500 mg/kg的2,4-DNT-3-SO_(3)^(−)和2,4-DNT-5-SO_(3)^(−)的降解率分别在第12天和第3天达到100%。液相色谱–质谱(LC-MS)分析表明,DNTS中的硝基被转化为氨基。鞘氨醇单胞菌X2具有广谱降解特性,不仅可以降解DNTS,还可以降解2,4,6-三硝基甲苯(TNT)、二硝基甲苯(DNT)和硝基甲苯(MNT)等多种硝基芳香族炸药,为TNT红水污染土壤生物修复提供可能性。Due to the shortage of bacteria resources for bioremediation of TNT red water polluted soil,a 2,4-dinitrotoluene sulfonates degrading strain X2 was isolated from a commercial microbial consortium.Based on the 16S rDNA sequence analysis and the physicbiochemical characteristics,strain X2 was identified as Sphingobium sp..The growth experiments were carried out to optimize the growth condition of X2.The biodegradation mechanisms of 2,4-DNT-3-SO_(3)^(−)and 2,4-DNT-5-SO_(3)^(−) by X2 and its biodegradation ability towards other niro-aromatic explosives were investigated.The results showed that the optimum growing conditions for X2 were 30℃,pH=7 and 1%salinity.The removal efficiencies of 2,4-DNT-3-SO_(3)^(−) and 2,4-DNT-5-SO_(3)^(−) reached 100% after 12 and 3 days,respectively.Liquid chromatography-mass spectrometry analysis showed that the nitro groups in dinitrotoluene sulfonates were transformed to amino groups.Sphingobium sp.X2 has broad-spectrum degradation characteristics.It can degrade not only dinitrotoluene sulfonates,but also other nitroaromatic pollutants like 2,4,6-trinitrotoluene,dinitrotoluene and mononitrotoluene,providing possibilities for TNT red water bioremediation.

关 键 词：菌株分离鉴定 鞘氨醇单胞菌X2 2 4-二硝基甲苯磺酸盐 微生物降解 

分 类 号：X172[环境科学与工程—环境科学]