刍议质谱技术发现新型蛋白质翻译后修饰研究在化学与生物学教学中的应用  

Discussion on the Application of Novel Protein Post-Translational Modification Discovered by Mass Spectrometry in Chemistry and Biology Education

作　　者：王志鹏 张璇[4] 车子良[5] 蒋振雄 马新雨 Zhipeng AWang;Xuan Zhang;Ziliang Che;Zhenxiong Jiang;Xinyu Ma(Division of Genetics,Department of Medicine,Brigham and Women’s Hospital,Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School,Boston,MA 02115,USA.;Department of Chemistry,Texas A&M University,College Station,TX 77840,USA.;Department of Chemistry,Tsinghua University,Beijing 100084,China.;School of Life Sciences,Tsinghua University,Beijing 100084,China.;Department of Mathematics,Harvard University,MA 02138,USA.;Department of Biology,Texas A&M University,College Station,TX 77840,USA.)

机构地区：[1]Division of Genetics,Department of Medicine,Brigham and Women’s Hospital,Department of Biological Chemistry and Molecular Pharmacology,Harvard Medical School,Boston,MA 02115,USA. [2]Department of Chemistry,Texas A&M University,College Station,TX 77840,USA. [3]清华大学化学系,北京100084 [4]清华大学生命学院,北京100084 [5]Department of Mathematics,Harvard University,MA 02138,USA. [6]Department of Biology,Texas A&M University,College Station,TX 77840,USA.

出　　处：《大学化学》2021年第6期185-193,共9页University Chemistry

摘　　要：随着质谱技术的不断发展,质谱技术已经被广泛应用于化学、材料、生命科学等众多领域,特别是其在生物化学与化学生物学领域的应用极大扩展了研究人员对于蛋白质翻译后修饰的认知。本文从基本的质谱原理入手,着重探讨了质谱技术在以组蛋白为模式蛋白的赖氨酸翻译后修饰的发现过程,并展示如何将基础有机化学和生物化学知识融入前沿科学研究。本文通过梳理不同的研究逻辑下质谱技术在蛋白翻译后修饰的研究中发挥的作用,旨在引导学生建立起将基础教学内容与实际应用相联系的意识及思维模式。Rapid development of mass spectrum technology(MS)brings about its wide applications in many fields such as chemistry,material science and life sciences,and its application in biochemistry and chemical biology have been greatly expanding the knowledge of novel protein post-translation modification(PTM).This manuscript starts from the basic principles of mass spectrum and later elaborates the application of MS in discovering lysine posttranslation modifications over histones as model proteins,showing a path incorporating fundamental organic chemistry and biochemistry knowledge into cutting-edge researches.By analyzing roles of mass spectrum throughout posttranslation modification studies under different research logistics,this manuscript was aimed to help establishing a way of thinking that associates fundamental chemistry and biology education with practical applications for students.

关 键 词：质谱技术 串联质谱 翻译后修饰 生物化学 

分 类 号：G64[文化科学—高等教育学] O6[文化科学—教育学]