环状RNA circEIF6在肝细胞癌中的表达及功能研究  

Expression and function of circRNA circEIF6 in hepatocellular carcinoma

作　　者：王乙钦 李顺 曾小芳 马志立 许丽霞[1,2] 匡铭[1,2,3] Wang Yiqin;Li Shun;Zeng Xiaofang;Ma Zhili;Xu Lixia;Kuang Ming(Institute of Precision Medicine,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China;Cancer Center,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China;Department of Liver Surgery,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou 510080,China)

机构地区：[1]中山大学附属第一医院精准医学研究院,广州510080 [2]中山大学附属第一医院肿瘤中心,广州510080 [3]中山大学附属第一医院肝外科,广州510080

出　　处：《中华普通外科学文献（电子版）》2021年第4期252-257,共6页Chinese Archives of General Surgery(Electronic Edition)

摘　　要：目的探讨环状RNA circEIF6在肝细胞癌中的表达及其对肝癌细胞增殖、侵袭和迁移能力的影响。方法从GEO数据库下载肝细胞癌与癌旁组织有差异表达的circRNA数据集GSE94508和GSE97332,选取在肝癌组织中高表达的circEIF6作为研究对象。选取2015年1月至2018年12月间中山大学附属第一医院确诊为肝细胞癌并行根治性切除术(术前均未经过放疗和化疗治疗)患者15例的肿瘤组织标本和配对的癌旁肝组织标本。采用qPCR检测circEIF6的表达;采用siRNA敲低circEIF6的表达,通过CCK8、EdU、平板克隆实验、Transwell实验方法检测肝癌细胞系Huh7细胞的增殖、侵袭与迁移能力的改变。结果筛选出肝癌差异表达circRNA共81个,对其中表达上调的73个circRNA对应的母基因进行GO分析。circEIF6在肝癌组织中的表达明显高于癌旁肝组织(P<0.05)。敲低circEIF6后,肝癌细胞系Huh7细胞增殖能力明显下降(P<0.05),Huh7细胞的侵袭能力和迁移能力明显下降(P<0.05)。结论circEIF6在肝癌组织中高表达,对肝癌细胞增殖、侵袭及迁移能力均有促进作用,值得进一步的机制探究。Objective To explore the expression of circRNA circEIF6 in hepatocellular carcinoma(HCC),and its effect on cell proliferation,invasion and migration in HCC.Methods Data sets GSE94508 and GSE97332 of circRNAs that differentially expressed between HCC and paracancerous tissues were downloaded from GEO database,and circEIF6,among circRNAs with higher expression in HCC tissues,was selected as the research object.A total of 15 cancer tissues and corresponding para-cancerous tissues were collected from HCC patients who underwent surgical resection in the First Affiliated Hospital of Sun Yat-sen University from January 2015 to December 2018.qPCR analysis was used to test the expression of circEIF6.Then,siRNAs were used to knock down the expression of circEIF6 in Huh7 cell line.CCK8 assay,EdU assay,plate clone formation assay,Transwell assay were conducted to analyze cell proliferation,invasion and migration in HCC.Results A total of 81 circRNAs differentially expressed in HCC were screened,and the corresponding maternal genes of 73 circRNAs with up-regulated expression were analyzed by GO.The expression of circEIF6 in liver cancer tissues was significantly higher than that in adjacent liver tissues(P<0.05).When circEIF6 was knocked down by siRNAs,the proliferation,invasion and migration abilities of Huh7 cells decreased significantly(P<0.05).Conclusion circEIF6 can promote cell proliferation,invasion and migration in HCC,and the related mechanism needs further study.

关 键 词：circRNA 肝细胞癌 SIRNA 增殖 侵袭 

分 类 号：R735.7[医药卫生—肿瘤]