牛蒡木屑栽培赤芝子实体多糖含量的检测及其配方筛选  

Determination of Polysaccharide Content in the Fruiting Body of Ganoderma lingzhi Cultivated with Burdock Sawdust and the Optimization of Its Formula

作　　者：姚凤腾[1] 董玉玮[2] 李胜男 YAO Fengteng;DONG Yuwei;LI Shengnan(Jiangsu Academy of Agricultural Sciences,Nanjing 210014,China;Xuzhou University of Technology,Food and Biology Institute of Technology,Xuzhou 221018,China;Huinan Rural Energy Technology Promotion Center,Tonghua 135100,China)

机构地区：[1]江苏省农业科学院,南京210014 [2]徐州工程学院食品与生物工程学院,徐州221018 [3]辉南县农村能源技术推广中心,通化135100

出　　处：《菌物研究》2021年第2期129-133,共5页Journal of Fungal Research

基　　金：江苏省农业科技创新与推广补助专项[KF(20)1011]。

摘　　要：为充分利用牛蒡和食药真菌资源,以不同体积比的牛蒡木屑为基质进行赤芝Ganoderma lingzhi栽培。测定不同条件下生长的赤芝子实体多糖含量,从而确定赤芝最优栽培条件。试验结果表明:当栽培基质中牛蒡与木屑体积比1∶4、温度28℃、固液比为20∶0时赤芝子实体多糖含量最高。用此栽培基质,在子实体形成期间,光照条件为暗光、p H=5~6、湿度90%~95%、培育45~60 d,子实体长势最好,形态最佳,其多糖含量可达23.26 mg/g。In order to make the best use of burdock and medicinal fungal fungi,the culture of Ganoderma lingzhi were carried on using different ratio of burdock solid medium.The optimal fermentation conditions of Ganoderma lingzhi were determined by observing the growth trend of mycelium and determining the content of polysaccharide.The results showed that the optimum conditions for culture of Ganoderma lingzhi were as follows:the volume ratio of burdock and sawdust was 1∶4,temperature was 28℃,solid-liquid ratio was 20∶0.During the period of fruiting body cultivation,the light condition was dark light,pH was 5-6,humidity was 90%-95%.After 45-60 days of cultivation,the cultivated fruiting bodies had the best growth and shape,and the polysaccharide content of fruiting bodies could reach 23.26 mg/g.

关 键 词：牛蒡 赤芝 固体培养 多糖 

分 类 号：S646[农业科学—蔬菜学]