母乳来源耐氧化双歧杆菌的筛选  

Screening of oxidation-resistant Bifidobacteriafrom breast milk

作　　者：胡鹏钰 康文丽 王鹏 于俊娟 瞿恒贤 彭灿 戴智勇 顾瑞霞 张臣臣[1,3] HU Pengyu;KANG Wenli;WANG Peng;YU Junjuan;QU Hengxian;PENG Can;DAI Zhiyong;GU Ruixia;ZHANG Chenchen(Jiangsu Key Laboratory of Dairy Biotechnology and Safety Control,Yangzhou University,Yangzhou 225127,China;Ausnutria Institute of Food and Nutrition,Ausnutria Dairy(China)Company Limited,Changsha 410299,China;College of Food Science and Engineering,Yangzhou University,Yangzhou 225127,China)

机构地区：[1]扬州大学江苏省乳品生物技术与安全控制重点实验室,江苏扬州225127 [2]澳优乳业(中国)有限公司澳优食品与营养研究院,长沙410299 [3]扬州大学食品科学与工程学院,江苏扬州225127

出　　处：《扬州大学学报（农业与生命科学版）》2021年第3期49-54,共6页Journal of Yangzhou University：Agricultural and Life Science Edition

基　　金：湖南省营养健康品工程技术研究中心(创新平台与人才计划)项目(2019TP2066);江苏省基础研究计划(自然科学基金)项目(BK20180910)。

摘　　要：双歧杆菌作为婴幼儿肠道的优势菌,是最常用的益生菌之一,但对氧气具有高度敏感性,因而双歧杆菌的分离、培养和应用面临较大困难。利用双歧杆菌选择培养基,从湖南采集的健康人的母乳中分离出19株乳酸菌。鉴定结果显示,其中7株双歧杆菌分别为1株长双歧杆菌(Bifidobacterium loungum)、2株两歧双歧杆菌(B.bifidum)和4株短双歧杆菌(B.breve)。长双歧杆菌S1和短双歧杆菌S3在pH 2.5的人工胃液中耐受性较好;短双歧杆菌S3在0.1%胆盐中存活率较高,达到75.50%;短双歧杆菌S3和S11在液体培养基中非厌氧条件下培养时的生长能力较强。短双歧杆菌S3有望作为一株优良的益生菌株,应用于双歧杆菌相关产品的开发。As a group of anaerobic bacteria in the human intestinal tract,bifidobacteria are highly sensitive to oxygen,which brings great difficulties to the separation,culture and application.In this study,19 strains of lactic acid bacteria were isolated from the breast milk of healthy humans collected in Hunan.Among them,7 Bifidobacterium strains included 1 strain of B.loungum,2 strains of B.bifidum and 4 strains of B.breve.The tolerance bifidobacteria to artificial gastric juice and bile salt was studied,and the oxygen tolerance of Bifidobacteria in liquid culture was measured.The results showed that strains S1 and S3 were well tolerated in pH 2.5 artificial gastric juice,S3 had a high survival rate of 75.50%in 0.1%bile salt,and S3 and S11 had a high oxygen tolerance in liquid culture.Strain S3 was expected to be an excellent beneficial strain and could be applied to the development of bifidobacteria-related products.

关 键 词：母乳 双歧杆菌 耐氧化 筛选 

分 类 号：TS201.4[轻工技术与工程—食品科学]