脑缺血/再灌注损伤小鼠生物学特性及稳定性  

Biological Characteristics and Stability of Cerebralischemia/Reperfusion Injury in Mice

作　　者：赵凯[1] 陈晓雪 陈雅婧[2] 贾宇臣[2] ZHAO Kai;CHEN Xiao-xue;CHEN Ya-jing;JIA Yu-chen(Huhhhot First Hospital, Hohhot 010030, China;Inner Mongolia Key Laboratory of Molecular Biology, School of Basic Medical Sciences, Inner Mongolia Medical University, Hohhot 010059, China)

机构地区：[1]呼和浩特市第一医院,呼和浩特010030 [2]内蒙古医科大学分子生物学研究中心,呼和浩特010059

出　　处：《科学技术与工程》2021年第21期8822-8827,共6页Science Technology and Engineering

基　　金：内蒙古自治区自然科学基金(2018MS08027);内蒙古医科大学实验室开放基金(2020ZN20)。

摘　　要：建立稳定的脑缺血/再灌注损伤小鼠模型,对于研究缺血性脑病具有至关重要的意义。为了建立一种易操作、成功率高、稳定性好的脑缺血再灌注损伤小鼠模型,使用70只无特定病原体(specific pathogen free,SPF)级健康雄性C57小鼠,随机分成假手术组(n=10)、颈总动脉(common carotid artery,CCA)进线组(n=30)及颈外动脉(external carotid artery,ECA)进线组(n=30),参照Zea Longa法,在线栓的选取、血管分离及处理、线栓插入方式等方面进行探讨,通过神经功能缺损评分、TTC染色检查脑梗死体积及苏木素/伊红染色观察脑组织形态学上的病理改变来分析确证模型成功率及稳定性。结果表明:ECA进线组神经功能缺损评分在2~3分比率占70.0%,CCA进线组占43.3%;ECA组和CCA组术后存活率分别为90%和66.7%,两组之间存在显著性差异(P<0.05);脑梗死体积和组织病理学,ECA进线组与CCA进线组之间均无差异(P>0.05)。采用颈外动脉进线方式与颈总动脉进线相比,脑梗死体积同样稳定且存活率更高。It is very important to establish a stable mouse model of cerebral ischemia/reperfusion injury for the study of ischemic encephalopathy.The model of local cerebral ischemia/reperfusion was prepared by middle cerebral artery occlusion(MCAO)in mice.Seventy specific pathogen free(SPF)grade C57 mice were randomly divided into 3 groups:sham group(n=10),external carotid artery(ECA)admission group(n=30)and common carotid artery(CCA)admission group group(n=30).With reference to Zea Longa method,the selection of online suppository,vascular separation and treatment,and the insertion method of thread suppository were discussed.The success rate and stability of the confirmative model were analyzed by neurological functional defect score,TTC staining for cerebral infarction volume and hematoxylin/eosin staining for pathological changes in brain histopathology.The scores of Nerve function evaluation score in ECA admission group accounted for 70.0%of 2~3 scores,while those in CCA admission group accounted for 43.3%.The survival rate of ECA admission group and CCA admission group was 90%and 66.7%,respectively,and there was a significant difference between the two groups(P<0.05).Cerebral infarction volume and histopathological findings indicated that there was no difference between the ECA admission and CCA admission groups.Compared with the CCA approach,the volume of cerebral infarction was also stable and the survival rate was higher.

关 键 词：脑卒中 缺血再灌注损伤 稳定性 线栓法 

分 类 号：R364[医药卫生—病理学]