肝双重动脉血供模型的建立与理论应用  

Establishment and theoretical application of a liver double artery blood supply model

作　　者：田全发 郑卫华 王子航 李朋飞 张俊晶[1,3] TIAN Quanfa;ZHENG Weihua;WANG Zihang;LI Pengfei;ZHANG Junjing(Hepatobiliary Surgery,Affiliated Hospital of Inner Mongolia Medical University,Hohhot 010030,China;Andrology of Urology,Fenyang Hospital of Shanxi Province,Fenyang 032200;Hepatobiliary Surgery,Huhhot First Hospital,Hohhot 010030)

机构地区：[1]内蒙古医科大学附属医院肝胆外科,呼和浩特010030 [2]山西省汾阳医院泌尿男科,山西汾阳032200 [3]呼和浩特市第一医院肝胆外科,呼和浩特010030

出　　处：《中国比较医学杂志》2021年第7期15-20,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(81560113);内蒙古自然科学基金重大项目(2015ZD(LH)01);内蒙古自然科学基金(2014MS0850);内蒙古自治区高等学校青年科技领军人才支持计划(NJYT-17-A15)。

摘　　要：目的建立稳定、成功率高的显微镜下大鼠70%肝切除+肝双重动脉血供的动物模型。方法纳入研究大鼠76只,分训练组40只、造模组36只。训练组模拟手术操作,优化围术期管理;模型组行显微镜下制备大鼠70%肝切除(肝左叶和中叶)+肝双重动脉血供手术,术后观察大鼠一般情况,计算造模成功率和1周存活率。结果通过训练组模拟操作,渡过学习曲线。造模组手术时间为(65.3±6.56)min,血管吻合时间(11.1±2.53)min。术中无明显副损伤,出血量(0.6±0.21)mL。1周后造模成功率88.9%(32/36);造模成功,大鼠术后状态良好。结论显微镜下制备大鼠70%肝切除+LDABS模型操作较为复杂,但通过一定时间显微技能训练后,通过对术式,特别是血管吻合及围手术期护理的改良及创新,使得该模型成功率高、可重复性强,为进一步研究其在肝再生、急性肝功能衰竭和肝移植等中应用奠定基础。Objective An animal model of 70%partial hepatectomy+liver dual arterial blood supply(LDABS)was established.Methods Seventy-six rats were divided into a training group(n=40)and a model group(n=36).In the model group,70%partial hepatectomy(left lobe and middle lobe of liver)+double artery blood supply surgery of the liver was performed under a microscope.The general status of rats was observed after surgery,and the success and 1 week survival rates were calculated.Results Through simulated surgery in the training group,the learning curve was crossed.The surgery duration of the model group was(65.3±6.56)min,and the vascular anastomosis time was(11.1±2.53)min.During the surgery,there were no obvious side injuries and less bleeding,of no more than 0.8 mL,was observed.One week later,the modeling success rate was 88.9%(32/36),and the rats were in good condition.Conclusions Surgery consisting of 70%hepatectomy+LDABS in rats under a microscope is relatively complicated.However,after a period of microsurgery training,the improvements and innovation of the surgical mode,especially vascular anastomosis and perioperative nursing,resultsed in a high success rate and strong repeatability of the model,laying the foundation for further research on its application in liver regeneration,acute liver failure,and liver transplantation.

关 键 词：门静脉动脉化 肝双重动脉血供 围手术期 血管吻合 肝切除 

分 类 号：R-33[医药卫生]