石斛人参蝙蝠蛾姜胶囊的功效成分测定与其对CI/F1小鼠免疫功能的调节作用研究  

Determination of functional components of Dendrobium-ginseng-paecilomyces batmoth-ginger Capsule and study on its immune function in CI/F1 mice

作　　者：程洁[2] 张成香 孙杰[1,2] 李世芬 王玉邦[1,2] 环飞 CHENG Jie;ZHANG Chengxiang;SUN Jie;LI Shifen;WANG Yubang;HUAN Fei(the Safety Assessment and Research Center for Drugs of Jiangsu Province,Nanjing 211166,China;School of Public Health Nanjing Medical University,Nanjing 211166)

机构地区：[1]江苏省医药农药兽药安全性评价与研究中心,南京211166 [2]南京医科大学公共卫生学院,南京211166

出　　处：《中国比较医学杂志》2021年第7期78-84,共7页Chinese Journal of Comparative Medicine

基　　金：江苏高校优势学科建设工程资助项目(苏政办发[2018]87号)。

摘　　要：目的测定石斛人参蝙蝠蛾姜胶囊中功效成分人参皂甙、多糖和腺苷的含量,研究石斛人参蝙蝠蛾姜胶囊对CI/F1(BALB/c×ICR)雌性小鼠的免疫调节作用。方法采用比色法测定人参皂甙和多糖,采用液相色谱法(HPLC)测定腺苷;采用小鼠经口灌胃分别给予260、530、1580 mg/kg的石斛人参蝙蝠蛾姜胶囊30 d后,观察其体重,测定其免疫器官指数、脾淋巴细胞增殖能力、小鼠迟发型变态反应,血清溶血素、自然杀伤细胞(NK细胞)活性、抗体生成细胞水平及腹腔巨噬细胞吞噬功能的变化。结果石斛人参蝙蝠蛾姜胶囊中功效成分的含量分别为人参皂甙:1.495 g/100 g、多糖:12.90 g/100 g和腺苷:73.8 mg/100 g;1580 mg/kg组可增强细胞免疫功能(脾淋巴细胞增殖能力和小鼠迟发型变态反应增强)、提高抗体生成细胞水平、提高小鼠巨噬细胞吞噬活性。结论在本实验条件下,石斛人参蝙蝠蛾姜胶囊有增强小鼠免疫功能的作用。Objective The ginsenosides,polysaccharides and adenosine contents in the Dendrobium-ginseng-paecilomyces batmoth-ginger Capsule were determined.To study the immune function effect of Dendrobium-ginseng-paecilomyces batmoth-ginger Capsule in CI/F1(BALB/c×ICR)female mice.Methods Ginsenoside and polysaccharide were determined by colorimetric method and adenosine was determined by High Performance Liquid Chromatography(HPLC).Dendrobium-ginseng-paecilomyces batmoth-ginger Capsule in distilled water was administered by oral gavage to female mice at a dose of 260,530 or 1580 mg/kg.After 30 d,the bodyweight,immune organ index,spleen lymphocyte proliferation ability,delayed type hypersensitivity,serum hemolysin,natural killer(NK)cell activity,antibody generation cellular level and the changes of macrophage phagocytic function in the abdominal cavity were evaluated for 10 female animals in each group at the end of the study.Results The ginsenoside,polysaccharide and adenosine contents in Dendrobium-ginseng-paecilomyces batmoth-ginger Capsule were 1.495 g/100 g,12.90 g/100 g and 73.8 mg/100 g,respectively.In the 1580 mg/kg group,the cellular immune function(spleen lymphocyte proliferation and delayed type hypersensitivity reaction)in the mice were enhanced,the level of antibody generation was elevated and the macrophage cellular and phagocytic activities were increased.Conclusions Under the conditions of this experiment,the Dendrobium-ginseng-paecilomyces batmoth-ginger Capsule enhanced the immune function in mice.

关 键 词：皂甙 多糖 腺苷 免疫功能 小鼠 

分 类 号：R-33[医药卫生]