葡萄糖-6-磷酸脱氢酶表达与肝细胞癌预后的关系  

Association of the expression of glucose-6-phosphate dehydrogenase with the prognosis of hepatocellular carcinoma

作　　者：刘明君 刘颖娟[1] 杨桂[1] 刘松梅[1] LIU Mingjun;LIU Yingjuan;YANG Gui;LIU Songmei(Department of Clinical Laboratory,Center for Gene Diagnosis,Program of Clinical Laboratory,Zhongnan Hospital of Wuhan University,Wuhan 430071,China)

机构地区：[1]武汉大学中南医院检验科/基因诊断中心/检验系,武汉430071

出　　处：《临床肝胆病杂志》2021年第8期1856-1860,共5页Journal of Clinical Hepatology

基　　金：国家自然科学基金(81772276)。

摘　　要：目的探究葡萄糖-6-磷酸脱氢酶(G6PD)基因在肝细胞癌(HCC)组织中的表达与患者预后的关系。方法收集2016年6月-2018年1月在武汉大学中南医院首诊的44例HCC患者癌组织及对应癌旁组织样本,RT-qRCR检测G6PD mRNA表达,比较癌组织和癌旁组织G6PD表达水平差异。按照G6PD基因表达水平的中位数将HCC患者分为G6PD高表达组(n=22)和G6PD低表达组(n=22),结合数据库数据进行分析,用Kaplan-Meier法绘制生存曲线,分析两组HCC患者总生存期和无进展生存期的差异。正态分布的计量资料2组间比较采用t检验,偏态分布的计量资料2组间比较采用Mann-Whitney U检验,计数资料2组间比较采用Fisher确切概率法;相关性分析采用Spearman法。结果肝癌组织中G6PD mRNA表达水平是癌旁组织的2.09倍,差异具有统计学意义(Z=-3.221,P=0.001)。G6PD高表达是影响HCC患者肝切除术后总生存期(HR=1.84,95%CI:1.30~2.61,P=0.000 52)和无进展生存期(HR=1.75,95%CI:1.27~2.42,P=0.000 54)的危险因素。在G6PD低表达组中淋巴细胞/单核细胞比值(LMR)明显高于G6PD高表达组(t=2.681,P=0.011),G6PD与LMR呈负相关(r=-0.439,P=0005)。结论肝癌患者癌组织中G6PD表达升高可能会引起LMR降低,G6PD表达可能与肿瘤微环境炎症有关,G6PD的高表达对HCC的预后评估具有一定的临床价值。Objective To investigate the expression of glucose-6-phosphate dehydrogenase(G6PD)in hepatocellular carcinoma(HCC)tissue and its association with prognosis.Methods HCC tissue samples and corresponding adjacent tissue samples were collected from 44 HCC patients who attended Zhongnan Hospital of Wuhan University from June 2016 to January 2018.RT-qRCR was used to measure the mRNA expression of G6PD,and the expression level of G6PD was compared between HCC tissue and adjacent tissue.According to the median of the mRNA expression of G6PD,HCC patients were divided into high G6PD expression group and low G6PD expression group and were analyzed with reference to the data in databases,and the Kaplan-Meier method was used to plot survival curves and investigate the differences in overall survival time and progression-free survival time between the two groups.The t-test was used for comparison of normally distributed continuous data between the two groups,and the Mann-Whitney U test was used for comparison of data with skewed distribution between the two groups;the Fisher’s exact test was used for comparison of categorical data between the two groups;the Spearman method was used to perform a correlation analysis.Results The mRNA expression level of G6PD in HCC tissue was 2.09 times that in adjacent tissue(Z=-3.221,P=0.001).High expression of G6PD was a risk factor for overall survival time(hazard ratio[HR]=1.84,95%confidence interval CI:1.30-2.61,P=0.00052)and progression-free survival time(HR=1.75,95%CI:1.27-2.42,P=0.00054)in HCC patients after hepatectomy.The low G6PD expression group had a significantly higher lymphocyte-monocyte ratio(LMR)than the high G6PD expression group(t=2.681,P=0.011),and G6PD was negatively correlated with LMR(r=-0.439,P=0.005).Conclusion Elevated expression of G6PD in HCC tissue may lead to a reduction in LMR in HCC patients,suggesting that G6PD expression might be associated with inflammation in tumor microenvironment.High expression of G6PD has a certain clinical value in evaluating the prog

关 键 词：癌 肝细胞 葡萄糖-6-磷酸脱氢酶 预后 

分 类 号：R735.7[医药卫生—肿瘤]