五株虫生真菌生物学特性及拮抗潜力初步研究  

Primary Study on Biological Characteristics and Antagonistic Potentiality of Five Strains of Entomogenous Fungi

作　　者：李敏[1] 杨帆[1] 刘春来[1] 刘兴龙[1] 王爽[1] 蒋希峰[1] 曹大为 李新民[1] LI Min;YANG Fan;LIU Chun-lai;LIU Xing-long;WANG Shuang;JIANG Xi-feng;CAO Da-wei;LI Xin-min(Plant Protection Institute of Heilongjiang Academy of Agricultural Science/Scientific Observing and Experimental Station of Crop Pest in Harbin,Ministry of Agriculture,Harbin 150086,China)

机构地区：[1]黑龙江省农业科学院植物保护研究所/农业部哈尔滨作物有害生物科学观测实验站,黑龙江哈尔滨150086

出　　处：《黑龙江农业科学》2021年第8期42-47,共6页Heilongjiang Agricultural Sciences

基　　金：黑龙江省应用技术研究与开发计划重大项目(GA19B104-5);黑龙江省农业科学院科研项目计划(2019YYYF037)。

摘　　要：为得到性状优良且具有生防潜力的虫生真菌,本试验对已获得的虫生真菌进行了基础生物学测定并对6种主要病原菌抑菌活性进行测定。结果表明:菌株FNS5和FYS3的最适生长温度分别为25~28℃和28~30℃,菌株FYS3的最适产孢温度较广,为20~30℃。菌株FNS5的最适产孢温度为20~25℃。48 h镜检菌株FNS5在25℃处理下萌发率最高为68%,与其他处理差异显著。菌株FNS5、FYS3均表现出广谱且较强的抑制作用,平皿对峙试验,金龟子绿僵菌FYS3对水稻稻瘟病的抑制率达47.13%,琼脂扩散法试验,金龟子绿僵菌FNS5、FYS3的代谢产物对番茄灰霉病菌具有拮抗作用,抑菌圈直径分别为(3.22±0.19)cm和(3.51±0.29)cm,是两株具有进一步研究价值的生防菌株。In order to obtain entomogenous fungi with excellent traits and potential for biocontrol,this experiment carried out basic biological assays on the obtained entomogenous fungi and the antibacterial activity of 6 main species pathogens.The results showed that the optimal growth temperature of strain FNS5 and FYS3 were 25-28℃and 28-30℃,respectively.The optimal sporulation temperature of strain FYS3 was wider,which was 20-30℃and strain FNS5 was 20-25℃.The germination rate of strain FNS5 under the 48 h microscopic examination was 68%at 25℃,which was significantly different from other treatments.Strains FNS5 and FYS3 both showed broad-spectrum and strong inhibitory effects.In the plate confrontation test,the inhibition rate of Metarhizium anisopliae FYS3 on Pyricularia grisea reached 47.13%.The agar diffusion test showed the metabolism of Metarhizium anisopliae FNS5 and FYS3 had an antagonistic effect on Botrytis cinerea,and the diameters of the inhibition zone were(3.22±0.19)cm and(3.51±0.29)cm,respectively.So they have further biocontrol research value.

关 键 词：虫生真菌 生物学特性 拮抗作用 

分 类 号：S43[农业科学—农业昆虫与害虫防治]