茴香叶片总RNA提取方法的比较  

Comparison of Total RNA Extraction Methods from Fennel Leaves

作　　者：王林 王晓敏 李霞 郭荔雯 孔维康 潘兵青 WANG Lin;WANG Xiao-min;LI Xia;GUO Li-wen;KONG Wei-kang;PAN Bing-qing(School of Agriculture,Ningxia University/Key Laboratory of Modern Molecular Breeding for Dominant and Special Crops in Ningxia/Ningxia Facility Horticulture(Ningxia University)Technology Innovation Center/Ningxia Modern Facility Horticulture Engineering Technology Research Center,Yinchuan 750021,China)

机构地区：[1]宁夏大学农学院/宁夏优势特色作物现代分子育种重点实验室/宁夏设施园艺(宁夏大学)技术创新中心/宁夏现代设施园艺工程技术研究中心,宁夏银川750021

出　　处：《黑龙江农业科学》2021年第8期48-51,共4页Heilongjiang Agricultural Sciences

基　　金：2012年度宁夏留学回国人员创新创业项目;宁夏回族自治区农业育种专项(NXNYYZ20150303)。

摘　　要：为促进茴香的分子生物学研究,以宁夏海原的茴香叶片为试验材料,分别采用CTAB法、CTAB改良法、CTAB水饱和酚法、Tris-硼酸法、异硫酸氰胍法、异硫酸氰胍改良法、Trizol法和Trizol改良法共8种方法提取茴香叶片RNA,比较其提取效果。结果表明:CTAB改良法提取的RNA质量最好,浓度为1481.9μg·μL-1,OD260/280为1.82,OD260/230为2.12,且得率较高,琼脂糖凝胶电泳显示条带清晰完整、无拖尾现象;Trizol法、Trizol改良法及异硫酸氰胍法提取的RNA质量较高,但完整性较差;CTAB法、CTAB水饱和酚法及异硫酸氰胍改良法提取RNA的效果较差;Tris-硼酸法提取的RNA质量最差。In order to promote the study of molecular biology of fennel,the leaves of fennel in Haiyuan of Ningxia were used as experimental materials,we used CTAB method,CTAB improved method,CTAB water saturated phenol method,Tris-boric acid method,cyanoguanidine isosulfate method,cyanoguanidine isosulfate improved method,Trizol method and Trizol improved method to extract RNA from fennel leaves,and compared their extraction effects.The results showed that the quality of RNA extracted by CTAB improved method was the best,and its concentration was 1481.9μg·μL-1,OD 260/280 and OD 260/230 were 1.82 and 2.12,respectively.The agarose gel electrophoresis showed that the bands were clear and intact without tailing.The quality of RNA extracted by Trizol method,improved Trizol method and cyanoguanidine isosulfate method was high,but the integrity was poor.CTAB method,CTAB water saturated phenol method and cyanoguanidine isosulfate improved method were poor in RNA extraction.The quality of RNA extracted by Tris-boric acid method was the worst.

关 键 词：茴香叶片 总RNA 提取方法 

分 类 号：R73[医药卫生—肿瘤]