短链脂肪酸酯生物合成研究进展  

Advances in the biosynthesis of short-chain fatty acid esters

作　　者：陆家声 章晓宇 蒋羽佳 章文明 信丰学 姜岷 LU Jiasheng;ZHANG Xiaoyu;JIANG Yujia;ZHANG Wenming;XIN Fengxue;JIANG Min(State Key Laboratory of Materials-Oriented Chemical Engineering,College of Biotechnology and Pharmaceutical Engineering,Nanjing Tech University,Nanjing 211816,China)

机构地区：[1]南京工业大学生物与制药工程学院材料化学工程国家重点实验室,南京211816

出　　处：《生物学杂志》2021年第4期1-11,共11页Journal of Biology

基　　金：国家重点研发计划项目(2018YFA0902200);国家自然科学基金项目(21978130,22008113);江苏省自然科学基金项目(BK20200683);国家博士后面上基金项目(2020M671465)。

摘　　要：通过微生物将生物质衍生的糖转化为短链脂肪酸酯是一种具有广泛前景的策略。归纳了利用微生物合成短链脂肪酸酯过程中的4种酶促途径及其涉及的酶,包括醇酰基转移酶(alcohol acyltransferase,AATs)、酯酶(esterases)、半缩醛脱氢酶(hemiacetal dehydrogenase,HADH)和Baeyer-Villiger单加氧酶(Baeyer-Villigermonooxygenase,BVMOs)。同时,综述近年来生物发酵法合成短链脂肪酸酯的研究进展,特别是乙酸乙酯和丁酸丁酯,包括利用醇酰基转移酶直接胞内合成短链脂肪酸酯和胞外补充脂肪酶合成短链脂肪酸酯。最后,以丁酸丁酯为例,提出基于生物发酵法合成短链脂肪酸的挑战与展望,以期为其他短链脂肪酸酯的生物合成提供借鉴。Microbial conversion of biomass-derived sugars into short-chain fatty acid esters is a promising strategy.This article summarized four enzymatic pathways and the enzymes involved in the synthesis of short-chain fatty acid esters by microorganisms,including alcohol acyltransferases(AATs),esterases,hemiacetal dehydrogenases(HADH)and Baeyer-Villiger monooxygenases(BVMOs).Meanwhile,recent research progresses in the synthesis of short-chain fatty acid esters by biological fermentation were also reviewed,especially ethyl acetate and butyl butyrate,including ester synthesis in vivo using alcohol acyltransferase and ester synthesis by extracellular esterification in vitro using lipase.Finally,taking butyl butyrate as an example,the challenges and prospects of the synthesis of short-chain fatty acids based on bio-fermentation were proposed,hoping to provide reference for the biosynthesis of other short-chain fatty acid esters.

关 键 词：短链脂肪酸酯 醇酰基转移酶 脂肪酶 丁酸丁酯 微生物共培养 

分 类 号：Q54[生物学—生物化学] Q55