翡翠绿葛仙米生活史特征研究  

Study on the life history characteristics of emerald green Nostoc sphaeroides

作　　者：王卫 易思富 田云[1,2] WANG Wei;YI Sifu;TIAN Yun(College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha 410128,China;Changde Yandi Biotechnology Co.,Ltd.,Changde 415000,China)

机构地区：[1]湖南农业大学生物科学技术学院,长沙410128 [2]常德炎帝生物科技有限公司,常德415000

出　　处：《生物学杂志》2021年第4期100-103,108,共5页Journal of Biology

基　　金：湖南省创新创业技术投资项目(2019GK5046);湖南省“100个”重大科技创新项目(2019KJ-063);常德市重大科技项目(常财企指[2018]50号);湖南农业大学“双一流”建设项目(SYL201802002)。

摘　　要：为探讨翡翠绿葛仙米生活史特征,固体培养藻殖段并定期取样制片,在显微镜下观察藻殖段发育过程中的形态变化。结果表明:在发育过程中,以藻殖段为发育起点的生活史主要包括藻殖段、微球体、藻丝体和异径藻体4个阶段。翡翠绿葛仙米主要以藻殖段繁殖和出芽繁殖进行营养增殖。此外,异形胞分化受藻细胞数量促进,且与出芽繁殖的“雏芽”形成和后期群间单连藻丝断裂有关,说明异形胞是葛仙米生长分化调控的关键靶点。在葛仙米制种过程中,需要关注异形胞对葛仙米生长分化调控的靶点效应。To explore its life history characteristics,solid culture of hormogonium and regular sampling and production,morphological changes were studied during the development of hormogonium under microscope.The results showed that the life history of the hormogonium was composed of four stages:hormogonium,the minimal colonies,filamentation of seriate colonies and the different diameter colonies.The vegetative proliferation of the emerald green Nostoc sphaeroides was mainly caused by hormogonium and germinal reproduction.In addition,the heterocyst differentiation was promoted by the number of algal cells,and it was related to the formation of‘embryonic bud’of germinal reproduction and the fracture of single algae filaments between groups in the later stage of budding.The results showed that hormogonium was the key targets of growth and differentiation regulation.In the process of seed production of Nostoc sphaeroides,it is necessary to pay attention to the effect of heterocyst on the regulation of growth and differentiation of Nostoc sphaeroides.

关 键 词：葛仙米 生活史特征 藻殖段 出芽繁殖 

分 类 号：Q945[生物学—植物学] S917.3[农业科学—水产科学]